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Guided by the emphasis on learning process, the educational reform has designed a "trinity" comprehensive evaluation system (quantitative clinical practice, in-class medical record analysis, and staged comprehensive written test) as the formative evaluation of the course. Through this assessment system, students' self-learning potential is stimulated, clinical skills practice is strengthened, and "taking exams to promote learning and taking exams to promote teaching" is realized. In the practice of teaching reform, it has been found that compared with the conventional teaching class, the students in the teaching reform class have higher participation and are more satisfied with the process assessment of the "trinity" comprehensive evaluation system.
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Objective To observe the effects of intra-articular injection of sinomenine on the expressions of vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) in articular cartilage and synovium of rabbit knee osteoarthritis models; To discuss its mechanism of action. Methods Totally 40 New Zealand white rabbits were randomly divided into 4 groups: control group, model group, hyaluronate group and sinomenine group. Model group, hyaluronate group and sinomenine group established knee osteoarthritis model by using intra-articular injection of papain enzyme and were treated with saline, sodium hyaluronate and sinomenine respectively for 30 d, except for the control group. After treatment, method of immunohistochemistry was used to detect the expressions of VEGF and NGF in rabbit articular cartilage and synovium. Results Compared with the control group, the expressions of VEGF and NGF in articular cartilage and synovium of model group significantly increased (P<0.01); compared with the model group, the expressions of VEGF and NGF in articular cartilage and synovium of sinomenine group significantly decreased (P<0.05, P<0.01); the expressions of VEGF and NGF in articular cartilage and synovium in sinomenine group were lower than hyaluronate group (P<0.05, P<0.01). Conclusion Intra-articular injection of sinomenine can down-regulate the expressions of VEGF and NGF in articular cartilage and synovium of rabbit knee osteoarthritis.
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Objective To observe the effects of intra-articular injection of sinomenine on morphology of synovium and cartilage as well as contents of MMP-13 and cartilage oligomeric matrix protein(COMP) in serum and synovial fluid of rabbit knee osteoarthritis model.Methods A total of 38 New Zealand white rabbits were randomly divided into 4 groups: control group, model group, hyaluronate group and sinomenine group. Model group, hyaluronate group and sinomenine group established knee osteoarthritis model by intra-articular injection of papain enzyme, and were treated with saline, sodium hyaluronate and sinomenine respectively except for control group. 5 weeks after treatment, all rabbits were sacrificed for HE staining and histological grading on cartilago articularis and synovium, and ELISA method was used to detect the contents of MMP-13 and COMP in rabbit serum and synovial fluid.Results Mankin's scores of articular cartilage and histological scores of synovium in model group were significantly higher than those of control group (P<0.01), and were markedly lower in sinomenine group than those of model group (P<0.01); the contents of MMP-13 and COMP in serum and synovial fluid of model group were significantly raised compared with control group (P<0.01), and were reduced obviously in sinomenine group compared with model group (P<0.05,P<0.01).Conclusion Intra-articular injection of sinomenine can reduce the levels of MMP-13 and COMP in serum and synovial fluid of rabbit knee osteoarthritis model, and improve synovial inflammation, as well as delay the degradation of articular cartilage.
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Objective:To observe the efficacy of warm needling in canicular days in treating knee osteoarthritis (KOA). Methods:Sixty-eight KOA patients were randomized into a treatment group and a control group, 34 in each group. The treatment group was intervened by warm needling, while the control group was by conventional acupuncture. The intervention started from the first day of the canicular days, once every day, 10 sessions as a treatment course. The improvement and therapeutic efficacy were evaluated after 3 courses of treatment. Results:The total effective rate was 91.2%in the treatment group versus 67.6%in the control group, and the difference was statistically significant (P Conclusion:Warm needling in canicular days can produce a better efficacy in treating KOA than conventional acupuncture.
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Objective: To observe theclinical effect of tuina reduction manipulation on leg length discrepancy and lumbosacral pain due to sacroiliac joint subluxation. Methods: A total of 60eligible cases were randomly allocated into an observation group and a control group, 30 in each group. Cases in the observation group were treated with conventional tuina plus reduction manipulation of sacroiliac joint subluxation; whereas cases in the control group were treated with conventional tuina plus acupuncture. The clinical effects were observed after 10 times of treatment. In addition, the relapse rates were observed 2 months after treatment. Results: The total effective rate in the observation group was 80.0%, versus 50.0% in the control group, showing a statistically significant difference (P Conclusion: Tuina reduction manipulation can obtain substantial therapeutic effect for leg length discrepancy and lumbosacral pain due to sacroiliac joint subluxation, coupled with a low relapse rate.