RÉSUMÉ
Objective:To establish an HPLC method for the determination of indomethacin and glucosamine hydrochloride in glu-cosomine and indomethacin enteric-coated tablets. Methods:A waters Bridge C18(250 mm×4.6 mm,5 μm) column was used with ammonium dihydrogen phosphate (the pH value was adjusted to 3.0 by phosphoric acid) (A) and acetonitrile (B) as the mobile phase with gradient elution,the flow rate was 0.7 ml·min-1,the detection wavelength was 195 nm,the sample volume was 20 μl and the column temperature was 30℃. Results: Glucosamine hydrochloride and indomethacin had a good linear relationship within the range of 0.030 0-1.500 8 mg·ml-1(r=0.999 1) and 0.010 3-0.513 0 mg·ml-1(r=0.999 9), and the average recovery was 98.3%(RSD=0.33%,n=6)and 99.9%(RSD=0.06%,n=6), respectively. Conclusion: The method is accurate, rapid, sensi-tive and specific,and can be used to control the quality of the product.