Résumé
OBJECTIVE: To establish a method for simultaneous detection of 3 kinds of polycyclic aromatic hydrocarbons(PAHs) including phenanthrene,anthracene and 3,4-benzo( a) pyrene in workplace air by high performance liquid chromatography( HPLC). METHODS: The phenanthrene,anthracene and 3,4-benzo( a) pyrene were collected in the air of the workplace using glass fiber filter paper. The membrane sample was added with 5. 00 m L of acetone,and the samples were extracted by ultrasonic wave for 70 mintues and eluted with acetonitrile-water gradient. Liquid chromatography-UV detector with tandem fluorescence detector was used for determination. RESULTS: The good linearity rang of phenanthrene,anthracene and 3,4-benzo( a) pyrene was 0. 050-1. 000 mg/L with the correlation coefficients ≥0. 999 5; the detection limits were 0. 010,0. 007 and 0. 008 mg/L,and the minimum detectable concentrations were 0. 130,0. 090 and 0. 110μg/m3 respectively( air collection of 375 L). The desorption efficiencies were 86. 10%-99. 20% of phenanthrene,88. 60%-96. 40% of anthracene,and 84. 80%-99. 60% of 3,4-benzo( a) pyrene,respectively. The within-run relative standard deviation( RSD) were 1. 29%-3. 25%,1. 90%-3. 61% and 1. 30%-4. 82% respectively,the between-run RSD were 2. 41%-4. 07%,2. 02%-5. 12% and 2. 08%-4. 77% respectively. The standard recovery rates were 95. 00%-106. 14% of phenanthrene,93. 14%-106. 50% of anthracene, and 92. 86%-105. 50% of 3,4-benzo( a) pyrene,respectively. The samples could be stored at 4 ℃ for 7 days. CONCLUSION: This method is simple,accurate and reliable for the simultaneous detection of phenanthrene,anthracene and 3,4-benzo( a) pyrene in the air of workplace.