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Journal of Experimental Hematology ; (6): 976-980, 2010.
Article Dans Chinois | WPRIM | ID: wpr-237612

Résumé

The study was aimed to investigate the effect of deriving hematopoietic cells from human embryonic stem cells (hESCs) by the erythropoietin gene-modified conditioned medium of human mesenchymal cells. The mesenchymal stem cells (MSCs) steadily expressing EPO were established by lentiviral system. The expression of exogenous EPO was detected by RT-PCR and Western blot. After suspension culture, hESCs developed into embryonic bodies (EBs). Then the EB cells were cultured in conditional medium. The hESCs-derived hematopoietic cells were analyzed by immunofluorescence, CFU assay and RT-PCR. The results indicated that the exogenous EPO successfully expressed in the EPO transfected MSCs (EPO/MSCs). The supernatant from EPO/MSCs increased CD34(+) cell population and the expression of globin, and enhanced colony forming unit incidence. These effects were obviously higher than that of control. It is concluded that the EPO gene-modified conditioned medium of human mesenchymal cells can induce the hESCs to differentiate into hematopoietic cells.


Sujets)
Humains , Techniques de culture cellulaire , Différenciation cellulaire , Milieux de culture conditionnés , Pharmacologie , Cellules souches embryonnaires , Biologie cellulaire , Érythropoïétine , Génétique , Pharmacologie , Système hématopoïétique , Cellules souches mésenchymateuses , Biologie cellulaire , Métabolisme , Organismes génétiquement modifiés
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