Dinâmica da população de coliformes durante a compostagem automatizada de dejetos líquidos de suínos / Population dynamics during composting of fecal automated pig slurry

Microrganismos presentes em dejetos de suínos podem contaminar o meio ambiente. Embora a compostagem seja preconizada como um método eficiente para reduzir este potencial poluidor dos dejetos, existem poucas informações de pesquisa sobre tal processo. O presente trabalho teve o objetivo de avaliar a eficiência da compostagem automatizada dos dejetos líquidos de suínos (DLS) na redução da população de coliformes, usados como indicadores de poluição fecal. Os DLS foram adicionados periodicamente, durante 106 dias, em substrato constituído pela mistura, em partes iguais, de maravalha e serragem. Foram efetuadas 14 adições de DLS, e em cada adição as leiras de compostagem eram revolvidas por meio de uma máquina especialmente desenvolvida para este fim. Foram avaliados dois tratamentos com três repetições, sendo um com e outro sem adição de ácido fosfórico aos dejetos, até pH 6,0. A adição de ácido visou reduzir as perdas de N por volatilização de amônia (NH3) durante a compostagem. A avaliação da população de coliformes foi feita pela técnica do número mais provável (NMP), com uso do caldo Fluorocult, incubado a 37ºC por 24h e posterior leitura em luz ultravioleta. A população de coliformes fecais não foi afetada pela adição de ácido fosfórico. O processo de compostagem automatizada foi eficiente na redução de coliformes fecais, cuja população original passou de 4,2x1010 para 1,2 x 105 ao final da compostagem (156 dias) sem adição de ácido e de 3,8x1010 para 2,3x104 na compostagem com adição de ácido. Essa remoção de coliformes fecais, promovida pela compostagem automatizada dos dejetos líquidos de suínos, corresponde a 99,99 por cento...

Microorganisms present in pig manure can contaminate the environment. Although composting is recommended as an efficient method to reduce the pollution potential of waste, there is little research information on this process. This study aimed to evaluate the efficiency of automated composting of pig slurry (PS) in reducing the population of coliforms, used as fecal pollution indicators. The PS was added periodically during 106 days in substrate, with a mixture, in equal parts, of wood shavings and sawdust. There were 14 additions of PS and at each addition the compost windrows were revolved through a machine especially developed for this purpose. Two treatments with three replications were evaluated, one with and one without the addition of phosphoric acid to the slurry up to pH 6.0. The acid addition aimed to reduce N losses through the volatilization of ammonia (NH3) during composting. Coliforms were evaluated by the technique of most probable number (MPN) using the Fluorocult broth, incubated at 37 ° C for 24 h and subsequent reading in ultra violet light. The population of fecal coliforms was not affected by the addition of phosphoric acid. The automated composting process was effective in reducing faecal coliforms, whose original population decreased from 4.2 x 1010 to 1.2 x 105 at the end of composting (156 days) without addition of acid and from 3.8 x1010 to 2,3 x104 in compost with added acid. This removal of faecal coliforms, promoted by automated composting of pig slurry, corresponds to 99.99 percent...

Autonomic modulation of heart rate of young and postmenopausal women undergoing estrogen therapy

The aim of the present study was to determine whether estrogen therapy (ET) reduces alterations of the autonomic control of heart rate (HR) due to hypoestrogenism and aging. Thirteen young (24 ± 2.6 years), 10 postmenopausal (53 ± 4.6 years) undergoing ET (PM-ET), and 14 postmenopausal (56 ± 2.6 years) women not undergoing ET (PM) were studied. ET consisted of 0.625 mg/day conjugated equine estrogen. HR was recorded continuously for 8 min at rest in the supine and sitting positions. HR variability (HRV) was analyzed by time (SDNN and rMSSD indices) and frequency domain methods. Power spectral components are reported as normalized units (nu) at low (LF) and high (HF) frequencies, and as LF/HF ratio. Intergroup comparisons: SDNN index was higher in young (median: supine, 47 ms; sitting, 42 ms) than in PM-ET (33; 29 ms) and PM (31; 29 ms) women (P < 0.05). PM showed lower HFnu, higher LFnu and higher LF/HF ratio (supine: 44, 56, 1.29; sitting: 38, 62, 1.60) than the young group in the supine position (61, 39, 0.63) and the PM-ET group in the sitting position (57, 43, 0.75; P < 0.05). Intragroup comparisons: HR was lower in the supine than in the sitting position for all groups (P < 0.05). The HRV decrease from the supine to the sitting position was significant only in the young group. These results suggest that HRV decreases during aging. ET seems to attenuate this process, promoting a reduction in sympathetic activity on the heart and contributing to the cardioprotective effect of estrogen hormones.

Comparison of anaerobic threshold determined by visual and mathematical methods in healthy women

Several methods are used to estimate anaerobic threshold (AT) during exercise. The aim of the present study was to compare AT obtained by a graphic visual method for the estimate of ventilatory and metabolic variables (gold standard), to a bi-segmental linear regression mathematical model of Hinkley's algorithm applied to heart rate (HR) and carbon dioxide output (VCO2) data. Thirteen young (24 ± 2.63 years old) and 16 postmenopausal (57 ± 4.79 years old) healthy and sedentary women were submitted to a continuous ergospirometric incremental test on an electromagnetic braking cycloergometer with 10 to 20 W/min increases until physical exhaustion. The ventilatory variables were recorded breath-to-breath and HR was obtained beat-to-beat over real time. Data were analyzed by the nonparametric Friedman test and Spearman correlation test with the level of significance set at 5 percent. Power output (W), HR (bpm), oxygen uptake (VO2; mL kg-1 min-1), VO2 (mL/min), VCO2 (mL/min), and minute ventilation (VE; L/min) data observed at the AT level were similar for both methods and groups studied (P > 0.05). The VO2 (mL kg-1 min-1) data showed significant correlation (P < 0.05) between the gold standard method and the mathematical model when applied to HR (r s = 0.75) and VCO2 (r s = 0.78) data for the subjects as a whole (N = 29). The proposed mathematical method for the detection of changes in response patterns of VCO2 and HR was adequate and promising for AT detection in young and middle-aged women, representing a semi-automatic, non-invasive and objective AT measurement.

Análise dos índices espectrais da variabilidade da freqüência cardíaca em homens de meia idade e mulheres na pós-menopausa / Analysis of spectral indexes for heart rate variability in middle-aged men and postmenopausal women

OBJETIVO: Analisar e comparar a variabilidade da freqüência cardíaca (VFC), em repouso, de homens e mulheres de meia-idade. MÉTODOS: Foram estudados 10 homens (54 ± 3,2 anos) e 14 mulheres na pós-menopausa (56 ± 2,6 anos) que não faziam uso de terapia hormonal. A freqüência cardíaca (FC) e os intervalos R-R foram obtidos a partir do eletrocardiograma, batimento a batimento, durante 8 minutos em repouso, nas posições supina e sentada. A VFC foi analisada no domínio da freqüência, usando a transformada rápida de Fourier, por meio da qual foram obtidas as bandas de baixa (BF) e alta freqüência (AF), as quais foram expressas em unidades normalizadas (AFun) e (BFun) e na razão BF/AF. Foram utilizados os testes estatísticos não-paramétricos de Mann-Whitney e de Wilcoxon, com nível de significância de alfa= 5 por cento. RESULTADOS: Na comparação intergrupo, as mulheres apresentaram maiores valores da banda AFun e menores valores da banda BFun e da razão BF/AF em relação aos homens, diferenças essas significativas (p<0,05). Na comparação intragrupo, não foram observadas diferenças significativas nos índices de VFC entre as posições supina e sentada para os 2 grupos estudados. CONCLUSÃO: Nossos resultados mostram uma maior modulação vagal e menor simpática no controle autonômico da FC para as mulheres em comparação aos homens de mesma idade, o que sugere que as diferenças autonômicas relacionadas ao gênero não se devem unicamente aos níveis hormonais de estrogênio, uma vez que as mulheres estudadas já se encontravam na fase pós-menopausa. Outros fatores podem estar contribuindo para essas diferenças.

OBJECTIVE: To analyze and compare heart rate variability (HRV) in middle-aged men and women under resting conditions. METHOD: Ten men (54 ± 3.2 years) and fourteen postmenopausal women (56 ± 2.6 years) who were not using hormonal therapy were studied. Heart rates (HR) and R-R intervals (iR-R) on a beat-to-beat basis were obtained from electrocardiograms over an eight-minute period under resting conditions, in the supine and sitting positions. The HRV was analyzed in the frequency domain by means of fast Fourier transforms and the low (LF) and high (HF) frequency bands were obtained and presented as normalized units (LFnu and HFnu) and the LF/HF ratio. Wilcoxon and Mann-Whitney non-parametric statistical tests were used, with the significance level set at 5 percent. RESULTS: Comparing between the groups, the women presented significantly higher HFnu and lower LFnu and LF/HF ratios than did the men (p<0.05). Comparing within the groups, no significant differences (p>0.05) were found in the HRV indexes between the supine and sitting positions for either study group. CONCLUSION: Our results show greater vagal modulation and lower sympathetic activity in autonomic heart rate control among women than among men of similar age. This suggests that the gender-related autonomic differences are not solely dependent on estrogen levels, since the women studied were already postmenopausal. Other factors may be contributing towards these differences.

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing mature oocytes (median = 368 and 5.7 ng/ml, respectively; P < 0.05). Progesterone, androstenedione and estrone levels were not significantly different between mature and immature oocytes. The application of the receiver-operating characteristic curve statistical approach to determine the best cut-off point for the discrimination between mature and immature oocytes indicated levels of 505.8 ng/ml for E2 (81.0 percent sensitivity and 81.8 percent specificity) and of 10.4 ng/ml for testosterone (90.9 percent sensitivity and 82.4 percent specificity). Follicular diameter was associated negatively with E2 and testosterone levels in FF. There was a significant increase in progesterone/testosterone, progesterone/E2 and E2/testosterone ratios in FF containing mature oocytes, suggesting a reduction in conversion of C21 to C19, but not in aromatase activity. The overall fertility rate was 61 percent but there was no correlation between the steroid levels or their ratios and the fertilization rates. E2 and testosterone levels in FF may be used as a predictive parameter of oocyte maturity, but not for the in vitro fertilization rate.

Positive correlation of serum leptin with estradiol levels in patients with polycystic ovary syndrome

Patients with polycystic ovary syndrome (PCOS) usually are obese, insulin resistant and hyperinsulinemic. The known association between leptin, obesity andinsulin action suggests that leptin may have a role in PCOS but this has only been addressed peripherally. This study was designed to assess the relationship between serum leptin and the anthropometric, metabolic and endocrine variables of obese (body mass index, BMI > or = 30 kg/mý) and non-obese (BMI <30 kg/mý) PCOS patients. Twenty-eight PCOS patients and 24 control women subdivided into obese and non-obese groups were evaluated. Leptin, androgens, lipids, gonadotrophins and insulin-glucose response to the oral glucose tolerance test were measured by radioimmunoassay in all participants. The assays were done all in one time. The areas under the insulin curve (AUC-I) and the glycemia curve were calculated to identify patients with insulin resistance. Mean leptin levels were not significantly higher in patients with PCOS compared to the control group (21.2 ñ 10.2 vs 27.3 ñ 12.4 ng/ml). Leptin levels were found to be significantly higher in the obese subgroups both in patients with PCOS (26.9 ñ 9.3 vs 14.1 ñ 7.0 ng/ml) and in the control group (37.3 ñ 15.5 vs 12.9 ñ 5.8 ng/ml). The leptin of the PCOS group was correlated with BMI (r = 0.74; P < 0.0001) and estradiol (r = 0.48; P < 0.008) and tended to be correlated with the AUC-I (r = 0.36; P = 0.05). Of the parameters which showed a correlation with leptin in PCOS, only estradiol and probably insulinemia (AUC-I) did not show a significant correlation with BMI, suggesting that the other parameters were correlated with leptin due to their correlation with BMI. Estradiol correlated with leptin in PCOS patients regardless of their weight.

Analise da modulacao autonomica do coracao durante condicoes de repouso em homens de meia idade e mulheres pos-menopausa / Analysis of heart autonomic modulation during resting conditions in middle-aged men and postmenopausal women

O controle autonomico do coracao pode ser investigado a partir da analise da variabilidade da requencia cardiaca (VFC). Sabe-se que o aparecimento de doencas cardiovasculares (DCV) em mulheres aumenta com a idade, principalmente apos a menopausa, quando o risco torna-se similar ao observado em homens. Assim, o objetivo deste trabalho e analisar e comparar a VFC de homens de meia-idade e de mulheres pos-menopausa em condicoes de repouso. Foram estudados dois grupos saudaveis e sedentarios, sendo 10 homens de meia-idade (52,6 +- 2,63 anos) e 10 mulheres pos-menopausa (56,8+-5,09 anos) que nao faziam uso de terapia de reposicao hormonal. A frequencia cardiaca e os intervalos R-R (iR-R) foram obtidos a partir de eletrocardiograma em tempo real, batimento a batimento, durante 6 minutos em condicoes de repouso, nas posicoes supina(S) e sentada(SE). Para analise da VFC foram calculados os indices RMSM e RMSSD dos iR-R em milissegundo(ms). Nao foram observadas diferencas estatisticamente significativas nos valores dos indices RMSM e RMSSD entre os homens e as mulheres e entre as posicoes supina e sentada de ambos os grupos. A reducao da VFC observada em ambos os grupos estudados sugere similar decrescimo da modulacao parasimpatica sobre o coracao, fato que pode contribuir para o aumento do risco de DCV observado nessa faixa etaria em ambos os sexos

Pro domain peptide of HGCP-Iv cysteine proteinase inhibits nematode cysteine proteinases

Cysteine proteinases (CPs) are synthesized as zymogens and converted to mature proteinase forms by proteolytic cleavage and release of their pro domain peptides. A cDNA encoding a papain-like CP, called hgcp-Iv, was isolated from a Heterodera glycines J2 cDNA library, expressed and utilized to assess the ability of its propeptide to inhibit proteinase in its active form. The hgcp-Iv cDNA sequence encodes a polypeptide of 374 amino acids with the same domain organization as other cathepsin L-like CPs, including a hydrophobic signal sequence and a pro domain region. HGCP-Iv, produced in Escherichia coli as a fusion protein with thioredoxin, degrades the synthetic peptide benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin and is inhibited by E-64, a substrate and inhibitor commonly used for functional characterization of CPs. Recombinant propeptides of HGCP-Iv, expressed in E. coli, presented high inhibitory activity in vitro towards its cognate enzyme and proteinase activity of Meloidogyne incognita females, suggesting its usefulness in inhibiting nematode CPs in biological systems. Cysteine proteinases from other species produced no noticeable activity.

Heart rate variability under resting conditions in postmenopausal and young women

The aim of the present study was to compare the modulation of heart rate in a group of postmenopausal women to that of a group of young women under resting conditions on the basis of R-R interval variability. Ten healthy postmenopausal women (mean Ý SD, 58.3 Ý 6.8 years) and 10 healthy young women (mean Ý SD, 21.6 Ý 0.82 years) were submitted to a control resting electrocardiogram (ECG) in the supine and sitting positions over a period of 6 min. The ECG was obtained from a one-channel heart monitor at the CM5 lead and processed and stored using an analog to digital converter connected to a microcomputer. R-R intervals were calculated on a beat-to-beat basis from the ECG recording in real time using a signal-processing software. Heart rate variability (HRV) was expressed as standard deviation (RMSM) and mean square root (RMSSD). In the supine position, the postmenopausal group showed significantly lower (P<0.05) median values of RMSM (34.9) and RMSSD (22.32) than the young group (RMSM: 62.11 and RMSSD: 49.1). The same occurred in the sitting position (RMSM: 33.0 and RMSSD: 18.9 compared to RMSM: 57.6 and RMSSD: 42.8 for the young group). These results indicate a decrease in parasympathetic modulation in postmenopausal women compared to young women which was possibly due both to the influence of age and hormonal factors. Thus, time domain HRV proved to be a noninvasive and sensitive method for the identification of changes in autonomic modulation of the sinus node in postmenopausal women

Vicilins (7S storage globulins) of cowpea (Vigna unguiculata) seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae) larvae

The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata) seed vicilins (7S storage proteins) bound to these structures suggested that C. maculatus-susceptible vicilins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vicilins in the appropriate sections with immunogold conjugates. These labeling sites coincided with the sites labeled by an anti-chitin antibody. These results, taken together with those previously published showing that the lower rates of hydrolysis of variant vicilins from C. maculatus-resistant seeds by the insect's midgut proteinases and those showing that vicilins bind to chitin matrices, may explain the detrimental effects of variant vicilins on the development of C. maculatus larvae

Eficácia e segurança de um novo sistema matricial para liberaçäo transdérmica de stradiol comparativamente ao sistema clássico de reservatório / An open, randomized assessment of the efficacy and safety of two transdermal estradiol patches for relief of climacteric symptoms

Estudo comparativo, aberto, randomizado, foi conduzido em 10 centros da América Latina (oito no Brasil, um na Argentina e um no Chile), para avaliar a eficácia e segurança de dois sistemas transdérmicos para liberação de estradiol. Um total de 182 pacientes foi aleatoriamente distribuído para receber o sistema matricial (Climaderm-Wyeth-Ayerst) ou sistema clássico do tipo reservatório (Estraderm TTS 50 - Ciba-Geigy). Os sistemas foram aplicados na região inferior do abdome em áreas alternadas, durante seis ciclos consecutivos de 28 dias. Os sistemas liberavam aproximadamente, 50gr de estradiol por dia. Em ambos os grupos houve, em relação ao pré-tratamento, decréscimo significativo do número diário de fogachos nos ciclos de um a seis, porém houve, em relação ao sistema matricial, melhora significativa nos ciclos 4 (p = 0,033) e 6 (p = 0,046). Houve também diferença significativa entre os grupos com relação à fraqueza nos ciclos 2 (p = 0,019) e 3 (p + 0,015), fadiga no ciclo 2 (p = 0,033), interrupções do sono no ciclo 6 (p + 0,048), nervosismo no ciclo 3 (p = 0,045) e escore total nos ciclos 2 (p = 0,030) e 3 (p = 0,041), a favor do sistema matricial e 18 ( 21,2 por cento) do grupo medicado com o sistema reservatório abandonaram o tratamento

Aumento da PRL plasmatica frente ao estimulo com TRH em pacientes normo e hiperprolactinemicas. Resposta clinica e laboratorial apos tratamento com bromoergocriptina. / Plasma PRL rise due to stimulation with TRH in normo and hyperprolactinemic patients. Clinical and laboratorial response after treatment with bromoergocriptine

Submeteram-se 19 pacientes (9 normo e 10 hiperprolactinemicas) ao teste de estimulacao com TRH, 200 microgramo EV.Observou-se que, quanto maior o nivel de PRL plasmatica, menor foi seu incremento maximo frente ao estimulo. As pacientes normoprolactinemicas apresentaram incremento superior a 280% nos niveis de PRL enquanto que todas as pacientes com tumor hipofisario (PRL > 100 mg/ml) tiveram resposta inferior a 100%. No grupo de pacientes hiperprolactinemicas, sem tumor radiologicamente detectado, observou-se tanto resposta positiva (incremento > 280%) como resposta negativa (incremento < 100%) ao teste. Nas pacientes com galactorreia, apos o tratamento com bromoergocriptina, por 30 dias, observou-se que, quanto maior o nivel inicial, maior o decrescimo maximo da PRL plasmatica. Tambem em todos os grupos, observou-se melhora clinica das pacientes tratadas com bromoergocriptina. Os autores recomendam o uso desta droga, principalmente nos casos de hiperprolactinemia sem tumor detectado, e justificam, inclusive, o seu uso em recidivas e tumores avancados de hipofise, como opcao terapeutica, sendo indubitavel sua acao sobre a diminuicao do tamanho dos adenomas

Correlacao entre o quadro clinico e os niveis sericos de prolactina em pacientes de clinica ginecologica.Estudo retrospectivo. / Correlation between clinical features and serum prolactin levels in patients of a gynecologic out-patient clinic. A retrospective study

Diante da crescente importancia dos estados hiperprolactinemicos atendidas nos ambulatorios do Departamento de Ginecologia, Obstetricia e Pediatria do Hospital das Clinicas da Faculdade de Medicina de Ribeirao Preto - USP. Para o grupo controle (grupo I), tomaram-se outras 71 pacientes normoprolactinemicas (PRL entre 5 e 50ng/ ml) atendidas no referido servico, com doencas variaveis. As pacientes hiperprolactinemicas foram divididas nos grupos II (PRL) entre 50 e 100ng/ml, n igual 27) e III (PRL maior que 100ng/ml,n igual 21).A amenorreia foi mais frequente nos grupos II e III, enquanto os outros I, em menstruais foram mais frequentes no grupo I. A galactorreia esteve presente em 17% das pacientes do grupo I, em 18,6% do grupo II e em 62% do grupo III, sendo que a forma espontanea predominou no grupo III. Diante dos dados encontrados, os autores sugerem a dosagem da PRL nos casos de amenorreia ou outros disturbios menstruais, galactorreia, esterilidade e hipotiroidismo, assim como a investigacao radiologica sistematica de hipofise nos casos de hiperprolactinemia. Propoe-se ainda um protocolo pratico de conduta na sindrome amenorreia e/ou galactorreia