Alginate production by azotobacter Vineland

The production of alginate by a mucoid strain of Azotobacter vinelandii was studied in batch culture. Highest alginate yield was obtained using Jarman N 2,-free mineral medium supplemented with 4% sucrose and the pH was initially adjusted to 8.0. Maximum alginate yield and productivity were achieved at the late growth phase [4 days old cultures], when each flask containing 100m1 medium was inoculated with 5% [V/V] bacterial suspension of 36h old cultures and shaked at 250rpm