[Study on follicular characteristics, hormonal and biochemical profile in norgestomet+PMSG treated acyclic buffaloes]

This research was conducted to study the follicular dynamics, hormonal, biochemical profile and fertility response in acyclic and norgestomet+PMSG treated acyclic buffaloes in summer. The study animals were divided into two groups: group I [control [n=8]: no treatment] and II [treatment group [n=15]]. In group II, seven animals were used for follicular biochemical and hormonal profile and eight animals for fertility studies following Crestar[registered sign] [Intervet, France] treatment [day 0: Crestar[registered sign] insertion; day 8: 500 IU PMSG; day 9: Crestar[registered sign] removal; day 11 AI]. Follicular fluid stradiol [E2] and progesterone [P4] in acyclic and pre-ovulatory follicle in study groups was significantly [P<0.01] higher than peripheral level. Peripheral E2 concentration, during pre-ovulatory period in group II was higher [P<0.05] than group I. Significant correlation between serum and follicular E2 was deduced [r=0.888; P<0.01] as significant difference in serum cholesterol content was shown between groups. Lower follicular total protein [P<0.05] in acyclic animals and higher follicular glucose [P<0.05] in treated group were concluded. Significant correlation [r=-0.770; P<0.05] was observed between follicular cholesterol and triglycerides. Follicular characteristics, post PMSG administration, differed significantly [0.83 +/- 0.20 vs 1.32 +/- 0.12; P<0.01] in all buffaloes exhibiting estrus, out of which four conceived. In conclusion, follicular hormonal and biochemical profile exhibits alteration in protein and glucose level between summer acyclic and treated buffaloes. However, peripheral E2 along with fertility response showed significant difference [P<0.01] between the study groups with significant correlation in E2, cholesterol and triglycerides between peripheral and follicular compartment

Optimization of process variables for enhanced lactic acid production utilizing paneer whey as substrate in submerged fermentation process

In the present study lactic acid production was enhanced by optimizing the three process variables viz; inoculum size, temperature and pH using three factor five level CCRD [central composite rotatable design] by Lactobacillus delbruckii under SMF [submerged fermentation process]. Paneer [dairy byproduct] whey was used as sole substrate for lactic acid production. Design Expert 8.0.2.0 software depicted that an optimum concentration of 8% [v/v] size of inoculum, 5.50 pH and 36.53°C temperature gave lactic acid and biomass yield of 5.61 g/L[-1] and 4.27 g/L[-1], respectively. Lactic acid production was scale up in 7.5 L bioreactor under optimized conditions and it gave lactic acid and biomass yield of 39.2 +/- 1.4 and 47.6 +/- 0.8 g/L[-1], respectively. micro[g], Y[P/S], Y[P/X] and productivity were found to be 0.14 h[-1], 0.66 g/g, 0.7 g/g and 1.98 g/L[-1] h, respectively. Leudking Piret equation deduced that lactic acid production was growth associated which varies from earlier reports. Lactic acid was characterized by FTIR [Fourier transform infrared spectroscopy] and HPLC

Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis

A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.

Studies on transition metal complexes of Schiff's Base derived from salicylaldehyde and 2-amino 4-phenyl oxazole

Recently a large number of work on heterocyclic transition metal complexes and their antifungal activity have been studied in this Laboratory and survey of literature revealed that no work has been done in the structural and fungicidal activity of transition metal complexes of Schiff's base derived from 2-amino 4-phenyl oxazole and salicylaldehyde. This note deals with the study of structural and fungicidal activity of transition metal complexes of Co [II], Cu [II], and Ni[II] with Schiff's base 2-amino-4-phenyl oxazole and salicylaldehyde. The newly synthesised complexes were also screened for their antifungal activity against different fungi viz; Phoma exiaua, Macrophomia phoseoli and Colletatrichum-capsici at 100, 50 and 20 ppm