Relaibility of salivary testosterone measurements in diagnosis of polycystic ovarian syndrome

The aim of this study was to evaluate the reliability of a non-invasive method [saliva] for determination or testosterone level in diagnosing cases of Polycystic Ovarian Syndrome [PCOS], and comparing it with serum free testosterone. Fifty women with PCOS and 20 normal women as control group were selected for the study. They all aged between 23-35 years. For all patients serum follicle stimulating hormone [FSH] and lutinizing hormone [LH] were assessed by chemiluminscent assay. Serum free testosterone [FT] was assayed by Radioimmunoassay [RIA] and salivary testosterone [ST] by ELISA technique. BMI was calculated for all participants and transvaginal sonography to determine ovarian morophology and ovarian volume. The present study showed a significant positive correlation between salivary testosterone, as measured by ELISA, and serum free testosterone measured by RIA [P=0.001 and r=0.52]. Using the receiver operator curve, salivary testosterone was found to be more sensitive than serum [FT] [84% VS 66%] in diagnosing PCOS patients. Determination of salivary testosterone is a reliable method to detect changes in the concentration of available biologically active testosterone in the serum. Salivary testosterone provides a sensitive, simple, reliable, non-invasive and uncomplicated diagnostic approach for PCOS

Salivary testosterone measurements in diagnosis of polycystic - ovarian syndrome

The aim of this study was to evaluate the reliability of a non- invasive method[saliva] for determination of testosterone level in diagnosing cases of Polycystic Ovarian Syndrome [PCOS], and comparing it with serum free testosterone. Fifty women with PCOS and 20 normal women as control group were selected for the study. BMI was calculated for all participants and transvaginal ultrasound to determine ovarian morphology, ovarian volume. Serum follicle stimulating hormone [FSH], lutinizing hormone [LH] were assessed for all patients, serum free testosterone [FT] was assessed by Radioimmunoassay [RIA] and salivary testosterone [ST] by ELISA kit. The present study showed a significant positive correlation between salivary testosterone, as measured by ELISA, and serum free testosterone measured by RIA [p=0.001 and r= 0.52]. Using the receiver operator curve, salivary testosterone was found to be more sensitive than serum [FT] [84% Vs 66%] in diagnosing PCOS patients. In conclusion, determination of salivary testosterone is a reliable method to detect changes in the concentration of available biologically active testosterone in the serum. Salivary testosterone provides a sensitive, simple, reliable, non-invasive and uncomplicated diagnostic approach for PCOS

Relation of urinary prostaglandin E2 levels to uteroplacental blood flow and renal functions in preeclampsia

The objective of this study was to investigate the association between urinary prostaglandin E2 [PGE2] levels and uteroplacental blood flow and to test whether PGE2 contributes to the vasospasm and altered renal functions in pregnancies complicated by preeclampsia. Serum and urine samples of 63 pregnant women [29 preeclamptic and 34 normotensive] were investigated by means of PGE2 levels and urea, creatinine and creatinine clearance values. To all participants of the study, uteroplacental blood flow was assessed by uterine artery Doppler sonography flow patterns. Data from preeclamptic patients were compared with normotensive pregnancies as controls. Mean urinary PGE2 levels were significantly lower in preeclamptic patients than in normotensive women [2.67 +/- 0.65 vs. 4.35 +/- 0.89 pg/g creatinine, respectively, P < 0.001]. Preeclamptic patients had significantly higher serum urea and creatinine concentrations, and significantly lower creatinine clearance values compared to normal controls. Preeclamptic patients also showed significantly higher mean uterine artery resistance index [RI] values than controls. Correlation analysis revealed a significant inverse correlation between urinary PGE2 levels and uterine artery RI values [r = -0.84, P < 0.001]. Prostaglandin E2 [PGE2] is a relevant mediator of uteroplacental blood flow and its deficiency suggests a possible role in the vasospasm of preeclampsia. PGE2 deficiency is of relevance to preeclampsia by leading to reduced uteroplacental blood flow and altered renal functions