Résumé
Objectives: To assess the suitability of local strain of rabbits as animal diabetic model
Methods: The study was conducted in the Department of Pharmacology PGMI Lahore. 25 rabbits of local strain were injected alloxan monohydrate in the marginal ear vein with mean BSR of 117.8 +/- 12.5 mg/dl
Results: A 22 rabbits survived and mean BSR was 288.31 +/- 9.4, 8 days after the induction of diabetes
Conclusion: Alloxan induced diabetic rabbits of local strain is a suitable option for pharmacological research in our set-up
Résumé
Objective: the study was designed to investigate the effect of aloe vera leaf gel extract on lipid profile parameters like total cholesterol, triglycerides and low density lipoproteins in alloxan induced diabetic rabbits
Material and Methods: the rabbits were made diabetic by injecting alloxan monohydrate and divided in two groups of eight each. Group A was treated with distilled water and Group B was treated with ethanolic extract of aloe vera leaf gel for 28 days. The pre-treatment and posttreatment level of lipid profile parameters were recorded and compared. Statistical analysis was done with help of independent t-test. The effects in two experimental groups were analyzed with help of paired t-test
Results: after 28 days of treatment the mean cholesterol level reduced to 80.9 from 97.1, the mean triglycerides reduced to 149.9 from182.6 and mean LDL reduced to 30.8 from 35.6 mg/dl in the aloe vera group. The mean HDL levels increased to 28.2 from 24.5 mg/dl
Conclusion: there was significant lowering of lipid profile parameters with aloe vera leaf gel extract in alloxan induced diabetic rabbits
Résumé
Objective: to evaluate the use of chicken ileum preparation for graded dose response relationship of acetylcholine
Material and Methods: the isolated chicken ileum was obtained from market and processed for pharmacological testing in Tyrode's solution. Graded dose response of acetylcholine on this preparation was studied and recorded
Results: the responses obtained by this tissue preparation were similar to those with rabbit ileum preparation which is usually used in pharmacology experimentations in our set-up
Conclusion: the chicken ileum can be used successfully in routine pharmacology experiments in place of rabbit ileum preparation as it is cheaper and easily available
Résumé
Objective: to evaluate the anti-diabetic activity of aloe Vera ethanolic extract in alloxan induced hyperglycemic rabbits as an alternate mode of treatment for type-2 diabetes
Methods: in the present experiment sixty six healthy male rabbits of local strain weighing 1.0-1. 7 kg were divided into 3 groups. They were injected intravenously with alloxan monohydrate according to body weight to induce diabetes. Baseline normal blood sugar level of all the rabbits was recorded. The rabbits turned diabetic within 1-2 weeks after injection of alloxan. A BSR level of= 250 mg/ di was criterion for diabetes. Group A, served as control and was treated with placebo [5 ml of distilled water daily]. Group 8 was treated with Metformin [135 mg/ kg body weight daily] and Group C was treated with ethanolic extract of aloe Vera [300 mg/ kg body weight daily]. Blood sugar levels were recorded as 0hrs, 2hrs and 4hrs readings on days 1, 14 and 28 of the treatment. The data was recorded in specially designed proforma and analyzed with the help of computer software SPSS version 16
Results: there was no significant change in the BSL in Group A [the control group, diabetic rabbits treated with 5 ml/ day of distilled water]. Overall, in a period of 28 days, there was 44.1 % reduction of mean BSL in Metformin group and 25.3% reduction of mean BSL in Aloe Vera group
Conclusion: the ethanolic extract of Aloe Vera leaf gel exhibited anti-diabetic activity in alloxan induced diabetic rabbits. Although antihyperglycaemic effect of Aloe Vera gel extract is lesser than that of Metformin but it has a gradual and sustained pattern