Improved purification and yield of antitetanic antiserum with the aid of caprylic acid

The aim of this study was to obtain highly purified antitetanic globulin by optimizing the conditions of extraction and purification. This was achieved through adjustment of the concentration of pepsin, the time of digestion, the value of pH and the concentration of caprylic acid. Our results indicate that the best conditions to obtain antitetanic antiserum are 0.5% caprylic acid, pH 3.3, 3.5 gm/l pepsin and 90 min for digestion