Résumé
Background: Glioma is the most common primary brain tumor with poor prognosis. Temozolomide [TMZ] has been used with irradiation [IR] to treat gliomas. The aim of the present study was to evaluate the cytotoxic and radiosensitizing effect of TMZ when combined with high-dose and high-dose rate of gamma irradiation in vitro
Methods: Two 'U87MG' cell lines and skin fibroblast were cultured and assigned to five groups for 24, 48, and 72 hours. The groups were namely, TMZ group [2000 micro M/L], IR group [5 Gy], TMZ plus IR group, control group, and control solvent group. MTT assay was applied to evaluate cell viability. Data were analyzed with SPSS 21.0 software using one-way ANOVA and Kruskal-Wallis test. P<0.05 were considered statistically significant
Results: The slope of growth curve U87MG cells in semilogarithmic scale was equal to 27.36+/-0.89 hours. The viability of cells was determined for different TMZ and IR treatment groups. In terms of cell viability, there were no significant differences between the control and control solvent groups [P=0.35] and between treated group by IR [5 Gy] alone and TMZ [2000 micro M/ml] alone [P=0.15]. Data obtained for the cell viability of combined TMZ plus IR in both cell lines compared to TMZ or IR treated group alone showed a significant difference [P=0.002]
Conclusion: The evaluation of cells viability showed that TMZ in combination with IR on glioma cells led to a significant radiosensitivity compared to IR alone