Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 4 de 4
Filtre
Ajouter des filtres








Gamme d'année
1.
Indian J Exp Biol ; 2006 Jul; 44(7): 580-3
Article Dans Anglais | IMSEAR | ID: sea-57169

Résumé

Erythrocytes are excellent models for the study of interactions of xenobiotics with biomembranes. Present work is designed to study the in vitro effects of some organophosphates (ethion, chlorpyrifos, dimethoate and monocrotophos) on rat erythrocytes. Treatment of erythrocytes with organophosphates resulted in decreased erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) activity, whereas activities of glutathione-s-transferase (GST) and glutathione reductase (GR) were increased. Reduced Glutathione (GSH) content of RBCs was decreased after treatment with the pesticides. Increased activities of GST and GR were due to induction of natural defense mechanism of erythrocytes against the toxicity of the pesticides. Membrane bound enzymes like acetylcholinesterase (AChE), Na(+)-K(+)-ATPase and Ca(2+)-ATPase were also inhibited. Altered activities of these enzymes along with decreased GSH content indicate increased oxidative stress in erythrocytes after treatment with organophosphates.


Sujets)
Animaux , Antioxydants/métabolisme , Antienzymes/toxicité , Érythrocytes/effets des médicaments et des substances chimiques , Humains , Mâle , Pesticides/toxicité , Organophosphates/toxicité , Rats , Rat Wistar
2.
Indian J Exp Biol ; 2005 Aug; 43(8): 728-31
Article Dans Anglais | IMSEAR | ID: sea-60433

Résumé

t-BHP induced oxidative stress and Ca2+ function impairment in fresh hepatocytes was studied in order to understand its role in cytotoxicity. Viability of hepatocytes by the release of lactate dehydrogenase and methyl thiazoletetrazolium reduction method alongwith malondialdehyde formation indicated oxidative stress in the hepatotoxic action of t-BHP.


Sujets)
Animaux , Antioxydants/pharmacologie , Survie cellulaire/effets des médicaments et des substances chimiques , Cellules cultivées , Hépatocytes/effets des médicaments et des substances chimiques , Peroxydes lipidiques/métabolisme , Mâle , Malonaldéhyde/métabolisme , Rats , Rat Wistar , 2-Hydroperoxy-2-méthyl-propane/toxicité
3.
Indian J Exp Biol ; 2004 Mar; 42(3): 292-6
Article Dans Anglais | IMSEAR | ID: sea-57420

Résumé

In vitro effects of various organophosphate pesticides (dimethoate, chlorpyrifos, ethion and monocrotophos) were studied on hemolysis, K+ leakage and lipid peroxidation in rat erythrocytes. All the four pesticides increased hemolysis and K+ leakage from erythrocytes, that was concentration and time dependent. On the contrary, there was decrease in lipid peroxidation in erythrocyte membrane. Effect of pesticides on lipid peroxidation could be due to pesticide itself abstracting protons or interacting with free radicals rather than polyunsaturated fatty acids (PUFA), thereby protecting the latter against peroxidation.


Sujets)
Animaux , Érythrocytes/effets des médicaments et des substances chimiques , Acides gras insaturés/métabolisme , Hémolyse , Insecticides/pharmacologie , Peroxydation lipidique , Mâle , Composés organiques du phosphore , Pesticides/composition chimique , Potassium/composition chimique , Rats , Facteurs temps
4.
Indian J Exp Biol ; 2002 Aug; 40(8): 963-6
Article Dans Anglais | IMSEAR | ID: sea-62370

Résumé

The effect of oral administration of acephate (360 mg/kg body weight), for 15 days, daily, was investigated on the erythrocytes of male rats. Activities of acetyl cholinesterase and glucose-6-phosphate dehydrogenase decreased, while those of glutathione-s-transferase and glutathione reductase increased. Decreased glutathione content and increased lipid peroxidation suggest that there was increased oxidative stress in the erythrocytes of treated animals. Increased cholesterol/phospholipid ratio in the erythrocyte membranes and morphological changes in RBCs (scanning electron microscopy studies) were observed in acephate treated animals. The results clearly suggest that acephate induced oxidative stress in erythrocytes leads to morphological changes.


Sujets)
Acetylcholinesterase/métabolisme , Administration par voie orale , Animaux , Membrane cellulaire/composition chimique , Cholestérol/métabolisme , Érythrocytes/effets des médicaments et des substances chimiques , Glucose 6-phosphate dehydrogenase/métabolisme , Glutathion/métabolisme , Glutathione reductase/métabolisme , Glutathione transferase/métabolisme , Insecticides/toxicité , Peroxydation lipidique/effets des médicaments et des substances chimiques , Mâle , Composés organothiophosphorés/toxicité , Stress oxydatif/effets des médicaments et des substances chimiques , Phospholipides/métabolisme , Rats , Rat Wistar
SÉLECTION CITATIONS
Détails de la recherche