Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria

The identification of mycobacteria is essential because tuberculosis (TB) and mycobacteriosis are clinically indistinguishable and require different therapeutic regimens. The traditional phenotypic method is time consuming and may last up to 60 days. Indeed, rapid, affordable, specific and easy-to-perform identification methods are needed. We have previously described a polymerase chain reaction-based method called a mycobacteria mobility shift assay (MMSA) that was designed for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) species identification. The aim of this study was to assess the MMSA for the identification of MTC and NTM clinical isolates and to compare its performance with that of the PRA-hsp65 method. A total of 204 clinical isolates (102 NTM and 102 MTC) were identified by the MMSA and PRA-hsp65. For isolates for which these methods gave discordant results, definitive species identification was obtained by sequencing fragments of the 16S rRNA and hsp65 genes. Both methods correctly identified all MTC isolates. Among the NTM isolates, the MMSA alone assigned 94 (92.2%) to a complex or species, whereas the PRA-hsp65 method assigned 100% to a species. A 91.5% agreement was observed for the 94 NTM isolates identified by both methods. The MMSA provided correct identification for 96.8% of the NTM isolates compared with 94.7% for PRA-hsp65. The MMSA is a suitable auxiliary method for routine use for the rapid identification of mycobacteria.

A terapia manual nos sinais e sintomasdo bruxismo do sono / The manual therapy on the signs and symptoms of sleep bruxism

O bruxismo do sono é uma parafunção inconscientecom apertamento e/ou ranger dos dentes durante o sono. Objetivos:Identificar os efeitos da terapia manual sobre os sinais e sintomascausados pelo bruxismo do sono. Métodos: Após a 12ª aplicação doprotocolo de tratamento proposto, foi realizada a avaliação comos mesmos critérios da avaliação pré-tratamento. Foi realizada aanálise quantitativa dos dados obtidos através dos testes estatísticosde Kolmogoroff-Smirnof, t de Student e de Wilcoxon. Resultados:Houve diferença significativa entre as médias pré e pós-teste paradesvio lateral direito, aperta/range, qualidade de vida e saúde. Nonível de frequência dos sintomas a fadiga muscular e cefaleia forammais relatadas, seguido da tensão muscular e dor no masseter. Nopós-tratamento apresentam cefaleia e dor no masseter, sem queixas,desgaste dental e tensão muscular como queixa principal. Conclusão:A terapia manual mostra-se eficaz no auxílio ao tratamento dossintomas do portador de bruxismo de sono, porém nos sinais nãoforam encontrados resultados significativos. Para melhor comprovação,acredita-se que seria necessário maior tempo de aplicaçãodo protocolo assim como amostra maior e ação multiprofissionalpara atender de maneira abrangente o bruxismo do sono em todosos seus aspectos...

The sleep bruxism is a parafunction with unconsciousclenching and /or grinding of teeth during sleep. Objectives:To identify the effects of manual therapy on the signs and symptomscaused by sleep bruxism. Methods: After the 12th session of thetreatment protocol, the assessment was conducted with the samecriteria as in the pre-treatment. We performed the quantitative dataanalysis using the statistical tests of Kolmogorov-Smirnov, Studentt test and Wilcoxon. Results: There were significant differences betweenmean pre-and post-test for right lateral, clench/grind, qualityof life and health. The most common symptoms reported weremuscle fatigue and headache, as well as masseter muscle tensionand pain. After treatment individuals reported that did not haveheadache and masseter pain, but tooth wear and muscle pain werethe main complaint. Conclusion: The manual therapy is effective inhelping to treat symptoms of patients with sleep bruxism, but inthe signs was not found significant results. In order to be confirmed,we concluded that a longer-term therapy would be needed toimplement the manual therapy protocol, as well as a larger sampleand multiprofesional action to treat sleep bruxism in all aspects...