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Journal of Korean Medical Science ; : 257-261, 1993.
Article Dans Anglais | WPRIM | ID: wpr-18734

Résumé

We report here several experiences of interphase cytogenetics, using fluorescence in situ hybridization (FISH) technique, for the detection of chromosome aberrations. FISH, using alpha satellite specific probes of 18, X, Y chromosomes, was done in interphase nuclei from peripheral blood of patients with Edwards' syndrome, Klinefelter's syndrome and Turner's syndrome with healthy male and female controls, respectively. The distributions of fluorescent signals in 100 interphase nuclei were well correlated with metaphase findings. Nowadays FISH plays an increasingly important role in a variety of research areas, including cytogenetics, prenatal diagnosis, tumor biology, gene amplification and gene mapping.


Sujets)
Adolescent , Adulte , Enfant , Femelle , Humains , Nouveau-né , Mâle , Noyau de la cellule/ultrastructure , Aberrations des chromosomes/physiologie , Zébrage chromosomique , Hybridation fluorescente in situ , Interphase/physiologie
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