Résumé
Stress is thought to impair immune function through emotional or behavioral manifestations thus the present study was done to assessed the effect of ethanolic extract of Butea frondosa [BF] leaves on behaviour, immunomodulatory activity and brain acetyl cholinesterase activity in normal and stress induced male rats. Neuroprotective effects of BF, doses [100,200,400mg/kg p.o] were measured by assessing the changes in the behaviour and the immunity of the rats. In stress control, the results indicated that the retention transfer latency, time spent in a closed arm, agglutination , total leukocytes counts [TLC], total paw edema ,size of spleen , decreased significantly [p<0.01] while glucose level, size of the kidney and the liver, AChE activity increased significantly [p<0.01] in comparison with normal control. In BF [200mg/kg] treated rats, the results indicated that the time spent in a closed arm [p<0.01], agglutination [p<0.01], TLC [p<0.01], total paw edema [p<0.05], size of spleen[p<0.01] , increased significantly while glucose level [p<0.01], size of the kidney and the liver [p<0.01], AChE activity [p<0.01] decreased significantly in comparison with stress control. This study therefore concluded that the ethanolic extract of BF [200mg/kg] showed a protective effect against the stress induced impaired immune system and the psychological disorders
Résumé
Lipopolysaccharide [LPS] induced oxidative stress and impairment of normal physiological function generally categorized by increased anxiety and reduced mobility. Therefore, the present study was to find out the effect Methanolic extract of Asparagus racemosus [MEAR] in lipopolysaccharide [LPS]-induced oxidative stress in rats. LPS-induced oxidative stress in rats was measured by locomotor activity by photoactometer test, anxiety with elevated plus maze test and also studied the oxidative stress markers, nitric oxide and cytokines. The obtained data shows that LPS markedly exhausted [p<0.001] brain-reduced glutathione [GSH], superoxide dismutase [SOD] and catalase [CAT] significantly increased [p<0.001] the level of malondialdehyde [MDA], nitric oxide and the activity of cytokines in the brain. MEAR supplementation resulted in normalization of brain GSH and CAT and SOD and decreases in the levels of MDA with reduction of nitric oxide and cytokines in the brain. The action of the extract at dose of 200 mg/kg was almost similar to the standard drug, quercetin [100mg/kg, p.o.]. These present study conclude that MEAR administration significantly [P<0.05] reduced LPS- induced oxidative-stress and intensely suggest that Asparagus racemosus Willd. is a functionally newer type of cerebroprotective agent