Study of miR-4770 as a diagnostic markers for colorectal cancer / 实用医学杂志

Objective To identify specific miRNAs from the differentially expressed miRNAs as diagnose marker for colorectal cancer (CRC). Methods The expression levels of miRNAs in CRC and the adjacent tissues were detected by qRT-PCR and the candidate miRNAs with statistic significance were selected. Receiver-operating-characteristic curve was performed to analyse the specificity and sensitivity of miR-4770. Results A total of 102 significantly dysregulated miRNAs were identified , and 92 of them were down-regulated , 10 of them were up-regulated. The specificity of miR-4770 to discriminate moderated differentiated CRC from adjacent normal tissues was 71.43%, with the sensitivity of 95.24% and area under cure of 0.952 4. Conclusion miR-4770 can be potentially served as a diagnostic and prognostic biomarker for CRC.

The expression level of miR-125a-5p in colorectal cancer with various differentiation / 肿瘤研究与临床

Objective To identify the expression level of miR-125a-5p in colorectal cancer (CRC) with various differentiation,and to investigate its diagnostic significance.Methods Real-time polymerase chain reaction was used to analyze the expression level of miR-125a-5p in tumors and paired normal tissues of different differentiated CRC.Receiver-operating-characteristic (ROC) curve was performed to evaluate the specificity and sensitivity of using miR-125a-5p as biomarkers to discriminate CRC patients from normal persons.Results Compared with normal tissues,miR-125a-5p was down-regulated in both high and moderate differentiation,miR-125a-5p could distinguish CRC from normal persons with high sensitivity and specificity,which were 80.00 ％ and 90.00 ％,respectively.Conclusions miR-125a-5p down-regulated in CRC indicates that miR-125a-5p contributes to tumorigenesis,and may be a potential biomarker for CRC.

Study on miR-490-5p and miR-363 as novel biomarkers for the diagnosis of colorectal cancer / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To identify dysregulated microRNA(miRNA) that can act as novel biomarker for colorectal cancer screening.</p><p><b>METHODS</b>Real time-polymerase chain reaction was used to detect the expression profile of miRNA in tumor and paired normal tissues, and the significant dysregulated miRNA was examined by non-paired t-test. Receiver operating characteristic(ROC) curve was performed to evaluate the specificity and sensitivity of miR-363 and miR-490-5p as biomarkers to discriminate colorectal cancer patients from normal person.</p><p><b>RESULTS</b>Seven-three dysregulated miRNAs were found in male samples, of which 6 miRNAs were up-regulated and other 67 miRNAs were down-regulated, while 42 dysregulated miRNAs were found in female samples, of which 5 were up-regulated and 37 were down-regulated in tumor tissues compared with normal tissues. Among above dysregulated miRNAs, 33 miRNAs had significant differences, besides, dysregulated expression level of 10 of 33 miRNAs was over 5 folds in male and female as well as mixed samples.</p><p><b>CONCLUSIONS</b>The expression pattern in female is different from that in male. miR-363 and miR-490-5p possess the potential in screening colorectal cancer patients from healthy people.</p>

Role of centromere protein H in human gastric cancer cell proliferation / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the role of centromere protein H (CENP-H) in the proliferation of human gastric cancer cells.</p><p><b>METHODS</b>RT-PCR and Western blot analysis were employed to examine the mRNA and protein expressions of CENP-H in 7 human gastric cancer cell lines and immortalized human gastric epithelial cells (GES-1). The cells were infected with the retrovirus vectors pMSCV-CENP-H or CENP-H-RNAi to establish stable cell lines with high CENP-H expression or CENP-H expression interference. MTT assay and colony formation assay were used to examine the changes in the cell proliferation after the infection.</p><p><b>RESULTS</b>CENP-H was over-expressed in gastric cancer cell lines AGS, BGC823, SGC-7901, MKN45, HGC27, MGC-803 and MKN28 at both mRNA and protein levels. The established AGS/CENP-H cell line with increased CENP-H expression showed enhanced proliferative activity, while the cell line MGC-803/CENP-H-RNAi with CENP-H expression interference showed an obviously lowered proliferation ability.</p><p><b>CONCLUSION</b>CENP-H promotes the proliferation of human gastric cancer cells, suggesting its important role in the occurrence and development of gastric cancer.</p>