Construction and functional analysis of EGFRvIII CAR-T cells co-expressing IL-15 and CCL19 / 生物工程学报

The aim of this study was to investigate the functional characteristics and in vitro specific killing effect of EGFRvIII CAR-T cells co-expressing interleukin-15 and chemokine CCL19, in order to optimize the multiple functions of CAR-T cells and improve the therapeutic effect of CAR-T cells targeting EGFRvIII on glioblastoma (GBM). The recombinant lentivirus plasmid was obtained by genetic engineering, transfected into 293T cells to obtain lentivirus and infected T cells to obtain the fourth generation CAR-T cells targeting EGFRvIII (EGFRvIII-IL-15-CCL19 CAR-T). The expression rate of CAR molecules, proliferation, chemotactic ability, in vitro specific killing ability and anti-apoptotic ability of the fourth and second generation CAR-T cells (EGFRvIII CAR-T) were detected by flow cytometry, cell counter, chemotaxis chamber and apoptosis kit. The results showed that compared with EGFRvIII CAR-T cells, EGFRvIII-IL-15-CCL19 CAR-T cells successfully secreted IL-15 and CCL19, and had stronger proliferation, chemotactic ability and anti-apoptosis ability in vitro (all P < 0.05), while there was no significant difference in killing ability in vitro. Therefore, CAR-T cells targeting EGFRvIII and secreting IL-15 and CCL19 are expected to improve the therapeutic effect of glioblastoma and provide an experimental basis for clinical trials.

Anticoagulants combined with calcium and vitamin E in the early intervention of hypertensive disorder complicating pregnancy patients / 中国基层医药

Objective To observe the clinical curative effect of anticoagulants combined with calcium,vitamin E in pregnancy induced hypertension patients at high risk for early intervention.Methods 113 patients with hypertensive disorder complicating pregnancy were selected as the observation subjects,according to the detection of prethrombotic state before pregnancy,they were divided into observation group one 38 cases,group two 43 cases,control group 32 cases.The observation group one was given intervention preconception,the group two was given intervention during pregnancy,the control group was given intervention when abnormal.The incidence rates of obstetric complication,intrauterine distress and neonatal asphyxia were compared among the three groups.Results The incidence rates of hypertension,obstetric complications,intrauterine distress and neonatal asphyxia in the group one were 5.26％,7.89％,5.26％,5.26％,those in the group two were 20.93 ％,9.3 ％,18.6％,20.93 ％,those in the control group were 43.75％,28.13％,43.75％,48.88％,the differences between the observation group one and control group showed significant differences(x2 =14.59,5.01,14.59,16.36,all P ＜ 0.05),the differences between the group two and the control group were statistically significant (x2 =4.49,4.54,5.60,5.68,all P ＜ 0.05),the incidence rates of hypertension,neonatal asphyxia between the observation group one and group two had statistically significant differences (x2 =4.22,4.22,all P ＜ 0.05).Conclusion Anticoagulants combined with calcium,vitamin E in the treatment of patients with hypertensive disorder complicating pregnancy,can effectively correct the adverse pregnancy outcomes,and improve the prognosis of perinatal infants,and early intervention is better.

Clinical observation of jiont analyesia in labor / 中国基层医药

Objective To evaluate the effect of nitrous oxide-inhale combined with lidocaine-injection by Ciliao and Uterine points and single therapy in labor analgesia,and the impact on maternal and child.Methods 400 cases were randomly divided into four groups.Observation group:100 cases take the therapy of Nitrous oxide-inhale combined with lidocaine-injection in Ciliao and Uterine points in the time of active period.Control group 1:100 cases take the therapy of gas mixture-inhale( 50％ Nitrous oxide and 50％ Oxygen) in the time of active period.Control group 2:100 cases take the therapy of lidocaine-injection in Ciliao and Uterine points in the time of active period.Control group 3:100 cases do not take any analgesia.Analgesic effects,labor time,mode of delivery,neonatal conditions,the amount of bleeding in every group were observed.Results There was statistically significant differences between the observation group with the control group 1,2,3 on analgesic effects ( x2 =48.513,P ＜ 0.05 ) labor time( F =782.88,1120.81,1104.57,all P ＜ 0.05 ),mode of delivery ( x2 =85.715,P ＜ 0.05 ),neonatal conditions,the amount of bleeding( F =422.66,P ＜ 0.05 ).Conclusion Combination therapy was more effective than the single treatment.

Therapeutic effect of different treatment progarams for epithelial ovarian cancer / 中国基层医药

Objective To explore different methods on the efficacy of epithelial ovarian cancer.Methods The patients with epithelial ovarian cancer,a total of 265 cases,who was treated were selected and divided into A,B groups.Its surgical,chemotherapy and efficacy were compared.It was basically the same between the two surgical.On chemotherapy,A group was given PAC program mainly,B group Was given TA program mainly.Results Diameter of residual tumor lesions>2 cm respectively accounted for 29.2%.14.2%.The difference of clinical remission rate,partial remission rate was not significant.Effective rates were respectively 51.7% and 81.4%(P<0.05).To group A,the survival rates of 1-year,2-years,3-years,4-years,5-years were respectively 90.2%,86.9%,62.7%,45.8%,32.7%:The median survival period and relapse-free survival time was respectively 37.9 months and 17.4 months.To group B,the survival rates of 1-year,2-years,3-years,4-years,5-years were respectively 94.3%、91.1%、68.7%、56.4%、44.2%;The median survival period and relapse-free survival time wag respectively 50.5 months and 32.2 months.The difference of 5-years survival rate,median survival,relapse-free survival of the two groups was statistically significant(P<0.01 or 0.05).Conclusions The size of postoperative residual disease and the chemotherapy im-paeted the efficacy of epithelial ovarian cancer.TC chemotherapy was superior to PAC chemotherapy.