Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 2 de 2
Filtrer
1.
Chinese Pharmaceutical Journal ; (24): 2154-2157, 2014.
Article de Chinois | WPRIM | ID: wpr-860121

RÉSUMÉ

OBJECTIVE: To determine the content of patchouli alcohol in Huodan pills (traditional Chinese medicine) by near-infrared (NIR) spectroscopy. METHODS: The content of patchouli alcohol in 147 batches of Huodan pills samples were determined by GC. NIR spectra of all samples were collected, then standard normal variate, first derivative and Norris derivative filter methods were used as spectral preprocessing tools, and at last, the calibration model of patchouli alcohol content was established by partial least squares regression. RESULTS: The correlation coefficients, the root-mean-square error of calibration, and the root-mean-square error of cross-validation of the calibration model for patchouli alcohol were 0.95509, 0.0315, and 0.04510, respectively; the root-mean-square error of prediction and the average recovery rate of validation samples were 0.0343 and 100.76%, respectively. CONCLUSION: The method is stable, accurate, and can be used to determine the content of patchouli alcohol in Huodan pills rapidly.

2.
Zhongguo Zhong Yao Za Zhi ; (24): 1971-1975, 2007.
Article de Chinois | WPRIM | ID: wpr-307552

RÉSUMÉ

<p><b>OBJECTIVE</b>To study the phylogeny relationship and molecular identification of 10 species from Huperzia (Huperziaceae) based on matK gene sequences data.</p><p><b>METHOD</b>Total DNA of nine species from Huperzia was extracted; matK gene sequence was amplified by PCR. PCR product was directly sequenced after purification.</p><p><b>RESULT</b>The chloroplast matK gene nucleotide sequences from 9 species of Huperzia species were sequenced. The matK gene nucleotide sequences length was 1 589 bp. Analysis with Huperzia lucidula matK gene nucleotide sequences (download from GenBank) and taking Lycopodiella cernua as outgroup, Maximum Parsimony, Neighbor-Joining analyses and genetic distances were conducted using MEGA 3.1 software. 35 variable sites and 35 parsimony informative sites have been found. Pairwise genetic distances among 10 species of Huperzia was 1.59% - 0.25%.</p><p><b>CONCLUSION</b>The results were consistent with the taxonomy in morphological of Huperzia. But H. longipetiolata and H. serrata were resolved into in different clade. There are 19 different sites of matK gene sequences between H. longipetiolata and H. serrata, the genetic distances is 0.121%. It is suggested H. longipetiolata should be as an independent species.</p>


Sujet(s)
ADN des chloroplastes , Génétique , ADN des plantes , Chimie , Génétique , Endoribonucleases , Génétique , Huperzia , Classification , Génétique , Données de séquences moléculaires , Nucleotidyltransferases , Génétique , Phylogenèse , Protéines végétales , Génétique , Plantes médicinales , Classification , Génétique , Réaction de polymérisation en chaîne , Analyse de séquence d'ADN , Spécificité d'espèce
SÉLECTION CITATIONS
DÉTAIL DE RECHERCHE