Comparison of chemical compositions between Dendrobium candidum and Dendrobium nobile / 中国医学科学院学报

<p><b>OBJECTIVE</b>To compare the chemical compositions between Dendrobium candidum (D. candidum) and Dendrobium nobile (D. nobile).</p><p><b>METHOD</b>Relative area and content of every chromatographic peak in the ammonic chloroform extracts of D. candidum were compared with those of D. nobile with high performance liquid chromatography-mass spectrometry.</p><p><b>RESULTS</b>The relative area of alkaloids accounted for 2.34% and 41.87% in D. candidum and D. nobile, respectively. The relative area of 25 identical compositions took up 97.12% in the total area of D. candidum and 50.09% in that of D. nobile, and contents of 23 composition were higher in D. candidum than those in D. nobile.</p><p><b>CONCLUSIONS</b>The quantity and contents of alkaloids are remarkably different between D. candidum and D. nobile. However, D. candidum has a higher quality than D. nobile in terms of the same chemical compositions.</p>

Identification of Panax notoginseng and its preparations by LC/MS / 药学学报

<p><b>AIM</b>To develop a method of identifying the existing of Panax notoginseng in products of traditional Chinese medicine compound Danshen.</p><p><b>METHODS</b>Total ion chromatograms (TIC) of Panax notoginseng, P. ginseng, and P. quinquefolius were obtained by means of LC/MS. Extracted ion chromatograms (EIC) posses m/z of 770, 800, 932, 946 and 1,108 of above-mentioned three herbs was compared between species. EIC 800 and 946 were selected as differentiation marks to distinguish P. notoginseng from the other two species. The EIC 800 and 946 of P. notoginseng were also compared to the EICs obtained by the same method from Chinese patent medicines of compound Danshen pellet, compound Danshen tablet, and compound Danshen injection. EIC 800 and 946 of P. notoginseng and its products possess similar peaks, relative retention time, and relative integral areas. Main chemical constitutes of P. notoginseng were also identified by using LC/MS/MS.</p><p><b>RESULTS</b>EIC 800 and 946 were obviously different between P. notoginseng, P. ginseng, and P. quinquefolius. Patent medicines of compound Danshen pellet, compound Danshen tablet, which consist of extractions from P. notoginseng, possess the characteristic EICs. The selected EICs were stable and reproductive.</p><p><b>CONCLUSION</b>EIC 800 and 946, which correspond to ginsenoside Rg1, Re, and their isomers, can be used as identifying mark of P. notoginseng to differentiate it from other herbs, and also can be used to tell apart P. notoginseng from other herb extractions in Chinese patent medicines of compound Danshen.</p>

Studies on second metabolites of an endophytic fungus (II) / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from the cultured mycelia of a fungus Cephalosporium which accelerate the growth of plant.</p><p><b>METHOD</b>The constituents were isolated by column chromatography and identified by advanced physical and spectral analysis.</p><p><b>RESULT</b>Eleven compounds including 3-isopropyl-6-(1-methylpropyl) piperazine-2,5-dione(I), choline sulfate(II), 2-[(2-hydroxy tetracosanoyl) amino]-1,3,4-octadecatriol(III) were isolated and identified.</p><p><b>CONCLUSION</b>Compound I, II were isolated from Cephalosporium genus for the first time.</p>