STUDIES ON THE FERMENTATION CONDITIONS OF ASPERGILLUS NIGER FS25 PRODUCING ?-GLUC ANASE / 微生物学通报

The optimum fermentation conditions of Asp.niger FS25 producing ?-glucanase was as follows,medium compositi on(g/100mL):barley flour 6,corn liquid 2,bran 0 8(NH 4) 2SO 4 0 4,FeSO 4 ?7H 2O 0 01,Na 2HPO 4?3H 2O 0 1,CaCO 3 0 5,MgSO 4?7H 2O 0 03;i nitial pH 5 0,cultivate temperature 32℃,50mL medium volume in 250mL triangle flask.The ?-glucanase activity of fermentation fluid reached 80 1u/mL,increa sing 84 1% contrasted to the initial.