Study on HPLC-FPS of Pueraria of different sources / 中国中药杂志

<p><b>OBJECTIVE</b>To make comparative study on HPLC-FPS of several kinds of Pueraria lobata and P. thomsonii from different sources.</p><p><b>METHOD</b>Kromasil C18 column was used, with mixture of acetonitrile and water as mobile phase in a gradient mode. The wavelength of measurement was 250 nm.</p><p><b>RESULT AND CONCLUSION</b>The fingerprints of P. lobata and P. thomsonii were obtained. This method can be used to identify P. lobata and P. thomsonii from different sources conveniently, and it may be practically valuable for the quality control of sample for P. lobata or P. thomsonii and its preparation.</p>

Effect of total flavonoids of turpinia arguta seen on immune function in adjuvant arthritis rats in vitro / 中国药理学通报

Aim To study the effect of the total flavonoids of turpinia arguta seen (TFS) on immune function of rats with adjuvant arthritis. Methods Adjuvant arthritis (AA) was induced on d0 by intradermal injection of Complete Freund′s Adjuvant (FCA), containing 10 mg heat-inactive BCG in 1ml paraffin oil. On d 28 after immunization, AA rats were killed by cervical decollation. Immune cells (splenocytes and peritoneal macrophages) were obtained, then cultured in vitro with TFS. Some immune indexes were detected respectively such as the splenocytes proliferation and the productions of IL-1 and IL-2 were measured by the method of cell proliferation, the production of prostaglandin E2 was determined by radioimmunoassay. Results Compared with normal groups, the response of splenocytes to ConA and LPS was lowered, IL-2 syn-thesis was decreased, and IL-1 and PGE_2 released from PM? were elevated. TFS (10~ -8 ~10~ -4 mg?L~ -1 ) not only increased splenocytes proliferation, which induced by ConA and LPS, and IL-2 production of splenocytes, but also reduced the elevated productions of IL-1 and PGE_2 released from peritoned macrophate in AA rats in vitro. Conculsion TFS could adjust abnormal immune function in AA rats.