Bioinformatics screening and analysis of prognostic genes in breast cancer / 实用肿瘤学杂志

Objective Bioinformatics techniques were used to analyze the key genes that affect the survival of patients with breast cancer,so as to provide theoretical basis for the prognosis evaluation and targeted therapy of breast cancer.Methods The dif-ferentially expressed genes between breast cancer samples and normal breast samples were screened by TCGA database,enriched and analyzed by gene ontology(GO)and Kyoto Encyclopedia of Gene and Genome(KEGG).The protein-protein interaction network was constructed and the key genes were screened.The Kaplan-Meier method was used to find and verify the genes that might be used as potential prognostic biomarkers for breast cancer,and to explore the correlation between prognostic target genes and molecular typing and staging.The Timer database was used to analyze the correlation between prognosis-related target genes and immune cell infiltra-tion.Results A total of 1,285 differentially expressed genes were screened,including 318 up-regulated genes and 967 down-regu-lated genes(|log2FC| ≥ 1,P＜0.05).Differentially expressed genes were mainly enriched in cytokine-cytokine receptor interac-tions,PI3K-AKT signaling pathway,cAMP signaling pathway,and so on.A total of 10 key genes(AURKB,CDC20,CCNA2,NCAPG,BUB1,TOP2A,BUB1B,CCNB1,CDK1,and KIF11)were screened from the protein interaction network.Among them,the ex-pression of CCNA2,NCAPG and BUB1 in breast cancer tissues were higher than those in normal tissues.Their high expression was as-sociated with the poor prognosis of patient's overall survival(P＜0.05),and was significantly associated with the molecular typing and staging of breast cancer.The results of immune infiltration showed a significant correlation between the expression of CCNA2,NCAPG,BUB1 and the infiltration of immune cells such as B lymphocytes,CD8+T lymphocytes,neutrophils,dendritic cells and other immune cells.Conclusion CCNA2,NCAPG and BUB1 may be key genes in the occurrence and development of breast cancer,and their high expression is related to poor prognosis of breast cancer patients,which can be used as potential biomarkers for the prognosis of breast cancer.

Endovascular Treatment of Iliac Limb Occlusion After Endovascular Repair of Abdominal Aortic Aneurysm / 中国微创外科杂志

Objective To explore the effect of endovascular re-operation on iliac limb occlusion(ILO)after endovascular aneurysm repair(EVAR).Methods Clinical data of 6 patients with ILO after EVAR from March 2018 to March 2023 were retrospectively analyzed.All the patients underwent ipsilateral femoral artery incision with Fogaty balloon thrombectomy and iliac artery balloon dilation,3 of which were combined with stent implantation and 1 of which with popliteal artery thrombectomy of Rotarex.Results The recanalization rate of the 6 patients with iliac branch occlusion was 100%(6/6).The median follow-up time was 24 months(range,6-48 months).The symptoms of lower extremity ischemia disappeared in 5 patients(Rutherford 0 grade).One patient had limb ischemia and dry gangrene(RutherfordⅢgrade).Conclusion It is necessary to carefully analyze the causes and choose personalized endovascular treatment plans for ILO after EVAR.

Sleep status of children aged 3-12 years in Shanghai / 中国学校卫生

Objective@#To investigate the sleep characteristics of children aged 3-12 years old in Shanghai, and to provide a reference for improving pediatric sleep health.@*Methods@#From April to June 2019, 16 542 children aged 3-12 years old were randomly selected from 31 kindergartens and 17 primary schods in Shanghai, and a cross sectional epidemiological survey was conducted using multi stage cluster sampling. The Children s Sleep Habits Questionnaire (CSHQ) was used to evaluate sleep characteristics.@*Results@#The bedtime routine of children in Shanghai was (21:27±00:36) and (21:46±00:46) on weekdays and weekends, respectively. Corresponding wake up times were (6:52±00:28) and (7:47±00:48), total sleep duration was (9.05±1.07) and (9.52±1.17)h, and the prevalence of inadequate sleep was 81.0% and 55.4%. The average score of sleep problems was (51.56±8.04), and the prevalence of disturbed sleep was 93.5%. With advancing age, the total sleep time of children in Shanghai decreased, and the difference in weekend weekday sleep patterns increased. Furthermore, two major sleep problems were observed, namely, short sleep duration and daytime sleepiness, which became increasingly more prominent. Girls usually went to bed later on weekdays and woke up later on weekends, and a greater difference in weekend weekday sleep patterns was observed. Moreover, psychological problems such as sleep anxiety were more serious among girls.@*Conclusion@#At present, children in Shanghai have insufficient sleep time and late bedtimes. Large differences in weekend weekday sleep patterns were observed, and various sleep problems were identified. Sleep related anxiety and differences in weekend weekday sleep patterns were particularly high among girls.

Prevalence of allergic diseases and associated factors among schoolaged children in Shanghai / 中国学校卫生

Objective@#To examine the prevalence of allergic diseases in schoolaged children from Shanghai and to explore related factors so as to produce epidemiological data regarding allergic diseases in children.@*Methods@#Multistage cluster sampling was used to carry out the study in Shanghai from April to June 2019. A total of 10 686 children aged 7-12 years from 17 primary schools participated in the survey. The International Study of Asthma and Allergies in Childhood (ISAAC)Scale was used to evaluate allergic diseases. Multivariate Logistic regression analysis was performed to analyze the related factors.@*Results@#The overall prevalence of allergic diseases among schoolaged children in Shanghai was 47.0%. A higher prevalence was observed among boys (50.4% vs 43.3% in girls, χ2=54.44, P<0.01). Common allergic diseases included asthma (13.9%), allergic rhinitis (18.2%), and atopic dermatitis (34.3%). The Logistic regression analysis showed that the common risk factors of asthma, allergic rhinitis and atopic dermatitis included the following:male gender (OR=1.52,1.44,1.22); mother has a bachelors degree or above (OR=1.26,1.77,1.84); family history of allergic diseases (OR=2.87,4.24,2.57); only child (OR=1.16,1.28,1.22); curtain cleaning frequency <1 time/month (OR=1.41,1.79,1.77); room not cleaned daily (OR=1.14,1.18,1.20); and dust exposure frequency ≥1 time/month (OR=1.45,1.56,1.42), all P<0.05. These three types of allergic diseases were also associated with unique risk factors that dependent on socialenvironmentalbehavioral factors.@*Conclusion@#Compared with previous data, the prevalence of allergic diseases among schoolaged children in Shanghai increased significantly in 2019. The related influencing factors involve multiple variables including demographics, environmental exposure and behavior, which warrant further exploration.