Résumé
Background: Having low-grade chronic inflammation such as elevated C-reactive protein [CRP], interleukin-6 [IL-6] and tumor necrosis factor-alpha [TNF- alpha] plays a crucial role in polycystic ovary syndrome [PCOS]. This study aimed at investigating the therapeutic effects of curcumin on IL-6, CRP and TNF- alpha and symptoms of polycystic ovary syndrome
Methods: In this research, 72 female adult Wistar rats were divided into control [n=12], PCOS [n=12] and curcumin-treated PCOS groups [n=48]. PCOS was induced by injection of estradiol valerate [2mg/kg - one-step]. PCOS rats were divided into control and experimental groups which received daily intraperitoneal injection of curcumin. After 60 days of syndrome induction, ovaries were collected for histological and immunohistochemical evaluations. Serum IL-6 and CRP was detected by the ELISA kit. Data were analyzed using In-Stat 3 via one-way analysis of variance [ANOVA] and p<0.05 was considered statistically significant
Results: Histological studies showed a significant reduction in thickness of theca layer and increase in the number of corpus luteum [CL] diameter in the curcumintreated group compared with the PCOS group; also inflammatory markers such as IL-6 and CRP significantly decreased in groups treated with curcumin compared with PCOS groups. Regarding immunohistochemical analysis, the expression of TNF- alpha in granulosa layer and follicular fluid of follicles and ovarian cysts in PCOS group was more than the control group's expression. However, expression of this factor in the ovaries treated with curcumin was decreased
Conclusion: This study showed that the anti-inflammatory and antioxidant effects of curcumin on PCOS may be due to its inhibitory effect on expression and levels of TNF- alpha, serum IL-6 and CRP
Résumé
Objective: Curcumin protects the liver against injury and fibrosis through suppressing hepatic inflammation, attenuating hepatic oxidative stress [OS], and inhibiting hepatic stellate cells [HSCs] activation. Non-alcoholic fatty liver disease [NAFLD] and polycystic ovary syndrome [PCOS] are considered as common metabolic disorders. Low-grade chronic inflammation with different markers, such as elevated C-reactive protein [CRP] and interleukin-6 [IL-6] levels, play a crucial role in PCOS. This study aimed to evaluate the therapeutic effects of curcumin on IL-6 and CRP levels as well as insulin resistance [IR] index on liver function in PCOS rats
Materials and Methods: In this experimental study, 90 adult Wistar rats were divided into control [n=18], sham [n=18], PCOS [n=18] and curcumin-treated PCOS groups [n=36]. PCOS group was injected subcutaneously with 2 mg estradio-valerate [E2V]. After 60 days, PCOS group was treated with curcumin [100 and 300 mg/kg body weight [BW]] for 14 days and anesthetized by chloroform. Blood and liver samples were collected for histological and serological analyses. Data were analyzed using In-Stat 3 via one-way analysis of variance [ANOVA]
Results: Histological and serological analyses showed a reduction in number of necrotic cells, IR index, as well as IL-6 and CRP levels in PCOS rats that were treated with various concentrations of curcumin
Conclusion: In this study, curcumin decreased liver inflammation by induction of insulin sensitivity and reduction of hepatic necrosis. Therefore, curcumin may be considered as protective factor against inflammatory state of PCOS
Résumé
Polycystic ovary syndrome [PCOS] is the most common cause of infertility and endocrinopathy in women due to lack of ovulation. Silymarin has anti-inflammatory properties and is a modulator of the immune system. In this experimental study, the effect of silymarinon PCOS- induced rats was studied. In this experimental study, 144 adult Wistar rats were divided into groups of control, sham, PCOS [S.C. injection of 2mg estradiol valerate/ rat, once] and treated with silymarin. After induction of PCOS during 60 days, 20, 50, 100, 200 and 300 mg/kg BW silymarin was injected intraperitoneally for 10 days. Sham and control groups received DMSO and no injection, respectively. All groups were anesthetized and the serum and ovary of groups were collected in order to investigate the histological and serologic changes. Data were tested using ANOVA in Instat software and P< 0.05 was considered significant. PCOs induction resulted in elevation of follicular cyst formation, abnormal follicular development and increasing of androgen compared with control rats. Silymarin attenuated ovarian cysts and thickness of theca layer and improved hormonal balance and follicle development in PCOS rats. Silymarin can reduce the histological and hormonal symptoms of PCOS, and has a protective effect on ovary. It seems that this potency is due to the antioxidant and anti-inflammatory effects of silymarin, which can reduce cyst counts and improve the development of ovarian follicles