Résumé
R. tsutsugamushi strains found in chiggers collected from 3 different sites throughout Thailand were antigenically characterized. Monotypic infections were observed in 76.7% of the chiggers. Karp was the most predominant strain, followed by TA716, TA763, TA686 and Kato. This study represents the first confirmed report of R. tsutsugamushi infection in 8 different chigger species in Thailand.
Sujets)
Animaux , Mites (acariens)/microbiologie , Orientia tsutsugamushi/classification , Sérotypie , Thaïlande , Trombiculidae/microbiologieRésumé
An epidemiological study in a mature oil palm estate in Peninsular Malaysia has demonstrated a low prevalence of R. tsutsugamushi infection in small mammals. The direct fluorescent antibody technique for assaying infections in chiggers proved more sensitive than mouse inoculation. Most infections in both chiggers and rodents were caused by the Karp strain.
Sujets)
Animaux , Animaux sauvages/immunologie , Anticorps antibactériens/analyse , Vecteurs de maladies , Technique d'immunofluorescence , Malaisie , Mites (acariens)/immunologie , Orientia tsutsugamushi/immunologie , Rodentia/immunologie , Fièvre fluviale du Japon/immunologie , Sérotypie , Trombiculidae/immunologieRésumé
Rickettsia tsutsugamushi organisms were identified in Giemsa and fluorescent antibody stained monocyte cell cultures derived from experimentally infected monkeys and dogs. The identification of organisms in monocyte cell cultures compared favorably with the standard technique of mouse inoculation.
Sujets)
Animaux , Colorants azurés , Chiens , Technique d'immunofluorescence , Haplorhini , Monocytes/microbiologie , Orientia tsutsugamushi/isolement et purification , Fièvre fluviale du Japon/microbiologieRésumé
Dog sera, collected from different communities throughout Selangor, Peninsular Malaysia, were investigated for the presence of antibodies to R. tsutsugamushi and R. typhi. Scrub typhus antibodies were present in animals from the rural areas only, whereas murine typhus antibodies were observed in equal numbers of dogs from both rural and metropolitan areas. Greater percentage of dogs from suburban areas had demonstrable antibody titers to murine typhus than from the urban area.
Sujets)
Animaux , Anticorps antibactériens/analyse , Maladies des chiens/épidémiologie , Chiens , Technique d'immunofluorescence/méthodes , Malaisie , Orientia tsutsugamushi/immunologie , Rickettsia typhi/immunologie , Fièvre fluviale du Japon/diagnostic , Typhus murin/diagnosticRésumé
A high prevalence of murine typhus was found in the human and rodent populations of Senaling, West Malaysia. In addition to 2 index cases which prompted the investigation, 45% of the humans and 35% of the rodents had IFA titers of 1/50 or greater. Serological studies provided evidence of recent infections in 3 human subjects. In a control group of Malaysian soldiers IFA titers of 1/50 or greater were found in only 13 (5%) of 265 sera tested.