Résumé
BACKGROUND: Mutations in the quinolone resistance-determining regions (QRDRs) of Acinetobacter baumannii DNA gyrase (gyrA) and topoisomerase IV (parC) are linked to fluoroquinolone (FQ) resistance. We developed a mismatched PCR-restriction fragment length polymorphism (RFLP) assay to detect mutations in the gyrA and parC QRDRs associated with FQ resistance in A. baumannii. METHODS: Based on the conserved sequences of A. baumannii gyrA and parC, two primer sets were designed for mismatched PCR-RFLP to detect mutations in gyrA (codons 83 and 87) and parC (codons 80 and 84) by introducing an artificial restriction enzyme cleavage site into the PCR products. This assay was evaluated using 58 A. baumannii strains and 37 other Acinetobacter strains that have been identified by RNA polymerase β-subunit gene sequence analysis.
Sujets)
Acinetobacter baumannii , Acinetobacter , Séquence conservée , DNA gyrase , DNA topoisomerase IV , DNA-directed RNA polymerases , Réaction de polymérisation en chaîne , Analyse de séquenceRésumé
The actual measurement of the body surface area generally take much trouble. And the existance of some interrelation among the height, weight and the others now lead to the calculation of the body surface area using a way of multipling these factors by a constant coefficient. After much investigation about a variety of calculation formula which have desingned by now we reached a conculusion that a from A=K<SUB>1</SUB>X+K<SUB>2</SUB>Y+Q (Howland-Dana from) is more desirable than the others, above all by applying X for the height (Hcm) and Y for the weight (Wg) the most desirable approximation can be got. From this point of view we achieved a new calculation formula for Japanease adult man and woman as follows. A=0.0901W+62.49H+266 for man and A=0.1309W+ 37.33H + 1799 for woman come as the result.