Fingerprinting of Fuzhisan by high-performance liquid chromatography with ultraviolet and evaporative light scattering detector / 南方医科大学学报

<p><b>OBJECTIVE</b>To establish a method for fingerprinting of Fuzhisan (FZS, a traditional Chinese medicinal preparation) using high-performance liquid chromatography with ultraviolet and evaporative light scattering detector (HPLC-UV/ELSD) to allow simultaneous determination of 5 major constituents in the preparation.</p><p><b>METHODS</b>HPLC-UV/ELSD analysis was performed on water AlltechC18 column (5 microm, 4.6 mm x 250 mm) with a mixture of acetonitrile (A) and 0.1% acetice acid water (B) as the mobile phase. The solvent A gradient for elution was 0, 12%; 25, 20%; 30, 20%; 75, 30%; 105, 40%; 120, 80%; 130, 12%, with the flow rate of 1.0 ml/min; and the column temperature at 30 degrees . The detective wavelength was 335 nm, drift tube temperature was 80 degrees , pressure of nebulizer gas was 25 psi. The similarities between the HPLC-UV/ELSD fingerprints of the 12 extracts were calculated using similarity evaluation software.</p><p><b>RESULTS</b>The fingerprint of FZS was established and the 5 major constituents were identified. The complementarity between the fingerprints of UV and ELSD was analyzed, showing good correlation between 12 batches of FZS.</p><p><b>CONCLUSION</b>The method for fingerprinting can simultaneously characterize the main chemical constituents in FZS and allows stable, effective and comprehensive quality control and evaluation of FZS for a single sample.</p>

Expression of matrix metalloproteinase-9 and intercellular adhesion molecule-1 in the perihematomal tissues in human hypertensive intracerebral hemorrhage and its significance / 中华神经科杂志

Objective To investigate the relationship between the expression of matrix metalloproteinase-9 (MMP-9) and intercellular adhesion molecule-1 (ICAM-1) in the perihematomal tissues in human hypertensive,intracerebral hemorrhage (ICH) and brain edema formation following ICH.Methods Paraffin-embedded brain tissues of 39 human fatal cases of ICH from the perihematomal tissues,1—3 cm away from the margin of the hemorrhagic lesion,as well as tissues from the corresponding area at the opposite side as controls,were stained with HE and immunohistochemistry staining.The expressions of MMP-9 and ICAM-1 in the pefihematomal tissues were analyzed with the SPSS 11.5 system.Results ①With MMP-9 immunohistochemical staining positive capillaries in the perihematomal tissues were identified at 2 h ((1.2? 0.8)/HP).The number of MMP-9 positive capillaries began to rise at 5—10 h ((4.1?0.8)/HP) reaching the peak at 45—48 h ((10.6?1.4)/HP,P

Therapeutic action of Taurine in patients with cerebral infarction induced by hyperhomocysteinemia / 临床神经病学杂志

Objective To investigate the therapeutic action of Taurine in patients with cerebral infarction(CI)induced by hyperhomocysteinemia(Hhcy).Methods The 100 CI patients induced by Hhcy were randomly divided to two therapy groups.One group was administered Taurine,the other group was administered Folacin combined with Vitamine B12 for 8 weeks.Before and after treating,the serum levels of homocysteic acid(Hcy),Folacin and Vitamine B12 were detected,NIHSS and BI were evaluated.Results The serum levels of Hcy were significantly decreased after administering in both two groups(all P