Résumé
<p><b>OBJECTIVE</b>To perform an comparative proteome analysis of human papillomavirus-infected cervical specimens and to investigate different expressions between high- and low-risk genotypes.</p><p><b>METHODS</b>The cervical specimens were divided into two groups (cervical intraepithelial neoplasia group and condyloma acuminatum group) according to their genotypes. Using comparative proteome technology, high-risk human papillomavirus-infected cervical intraepithelial neoplasia, low-risk human papillomavirus-infected condyloma acuminatum, and normal cervical intraepithelial tissue were compared. The differential expression protein spots were identified by mass spectrometry.</p><p><b>RESULTS</b>Totally 26 differential spots were selected and analyzed, and 22 peptide mass fingerprints (PMF) maps were obtained by MALDI-TOF-MS. Eighteen proteins were preliminarily identified after searching the NCBInr database. The function information of these 18 proteins mainly involved cell metabolism, signal transduction, cell secretion, cell cytoskeleton construction, cell proliferation, and apoptosis.</p><p><b>CONCLUSION</b>The proteomic expressions after the cervical infection of high- or low-risk genotype of human papillomavirus are obviously different.</p>
Sujets)
Femelle , Humains , Dysplasie du col utérin , Métabolisme , Virologie , Col de l'utérus , Métabolisme , Condylomes acuminés , Métabolisme , Virologie , Génotype , Papillomaviridae , Génétique , Virulence , Infections à papillomavirus , Métabolisme , Virologie , Protéome , Métabolisme , Risque , Spectrométrie de masse MALDI , Maladies du col utérin , Métabolisme , VirologieRésumé
<p><b>OBJECTIVE</b>To compare the specificity and sensitivity of two genotyping approaches for human papillomavirus (HPV).</p><p><b>METHOD</b>HPV DNA was amplified and detected in clinical specimens by polymerase chain reaction in a pair of universal primers MY09/11, and then genotyped with either sequencing method or liquid chip hybridization method (luminex method).</p><p><b>RESULT</b>Sequencing method obtained precise genotyping results in single-type HPV infection, while luminex method obtained accurate genotyping results in multiple-type HPV infection.</p><p><b>CONCLUSION</b>A combined method using both sequencing and luminex method is suitable for the genotyping of HPV-infected specimens.</p>