Résumé
【Objective】 To compare the MicroRNA (miRNA) expression profiles in the subchondral bone between patients with Kashin-Beck disease and knee osteoarthritis and to conduct target gene prediction and biological function and signal pathway analysis so as to construct the regulatory network of miRNAs and target genes. 【Methods】 The subchondral bone samples were collected from four pairs of patients with Kashin-Beck disease and osteoarthritis. The total RNA was extracted and the miRNA array profiling was performed using Affymetrix miRNA 4.0 Array. The target gene prediction was performed using six different prediction programs including miRWalk. Cytoscape software was used to construct the regulatory network of significantly different miRNAs and predicted target genes; GO biological function and KEGG pathway enrichment analysis were performed based on target genes. 【Results】 A total of 124 miRNAs were significantly downregulated in the subchondral bone of patients with Kashin-Beck disease than that in patients with osteoarthritis. Target genes were predicted for eight miRNAs with a fold change more than 5, and 542 target genes and 1094 miRNA-mRNA regulatory relationships were obtained. Enrichment analysis of target genes in the regulatory relationship showed that these target genes were mainly involved in protein digestion and absorption, focal adhesion, PI3K-Akt signaling pathway and ECM-receptor interaction, and other signaling pathways. 【Conclusion】 Differentially expressed miRNAs in the subchondral bone of patients with Kashin-Beck disease and osteoarthritis may be used as the differential diagnostic markers between the two diseases. In addition, we preliminarily explored the potential molecular mechanism of changes in the subchondral bone of Kashin-Beck disease by bioinformatics analysis.
Résumé
Aiming at comparing the pre-operative and post-operative gait characteristics and therefore establishing post-operative rehabilitation guidance for patients with end-stage knee osteoarthritis (KOA) merged with varus deformity, this study captured the level walking and sit-to-stand trials of 9 patients with 3-dimensional motion analysis system and after which musculoskeletal multi-body dynamic analysis was conducted. The study indicated that the average range of motion (ROM) of the proposed-surgical knee was 24.4°-57.6° and that of the non-surgical knee was 22.5°-71.5°. The knee ROM of control group during level walking was 7.2°-62.4°. When the unilateral KOA patients stood up from chair to complete the sit-to-stand movement, the ground reaction forces (GRFs) symmetry was 0.72-0.85, which means that the non-surgical limb bear the majority of body weight. The GRFs of the bilateral KOA patients were smallest during the sit-to-stand movement. The strategy that the non-surgical limb dominates in loading bearing taken by the unilateral KOA patients to cover most post-operative daily activities could increase the risk of KOA among non-surgical side joints as a result of long-term excessive loading-bearing. The study, on kinematics and biomechanical characteristics of patients with KOA merged with varus deformity, could help to understand the pathogenesis of KOA merged with varus deformity from the perspective of biomechanics and to provide strong clinic guidance for the pre-operative evaluation, prevention and post-operative recovery for patients.
Résumé
0.05).Seroquel showed high incidence rate of drowsiness,dizziness and weight gain(26.1%,23.9%,17.3%),chlorpromazine showed high incidence rate of extrapyramidal side effect,pyknocardia and erect collapse(60.9%,39.1%,32.6%).CONCLUSIONS:There was no significant difference of curative effect for the treat-ment of schizophrenia between seroquel-treated group and chlorpromazine-treated group,but seroque showed low side effect and high safety.