Occurrence and antimicrobial susceptibility of enteric rods and pseudomonads isolated from the dental prostheses biofilm

ABSTRACT Aspiration of oral bacteria leads to cardiac and respiratory infectious diseases and dentures can act as a reservoir for pathogenic microorganisms. Objective: To determine the occurrence and the in vitro antimicrobial susceptibility of enteric rods and pseudomonads from the denture biofilm of 52 subjects at the Center for Dental Specialties of Sobral/ Ceara, Brazil. Material and Methods: Denture biofilm was collected and samples plated on MacConkey agar. The isolated bacterial colonies were identified using the BBL Crystal enteric/non-fermenter system. Antibiotic bacterial susceptibility was assessed by the disc diffusion method of amoxicillin, amoxicillin/clavulanic acid, doxycycline, tetracycline, tobramycin, imipenem, cefotaxime, and ciprofloxacin. The Minimum Inhibitory Concentration (MIC) of cefotaxime, tobramycin, doxycycline, imipenem, and ciprofloxacin was determined for 40 species by E-test. Results: 34 subjects (65.4%) harbored enteric rods in their prostheses. Klebsiella pneumoniae (26.5%), Escherichia coli (23.5%), and Enterobacter aerogenes (23.5%) were the most prevalent species. All organisms were susceptible to ciprofloxacin and most species were resistant to amoxicillin or amoxicillin/clavulanic acid, demonstrating variable sensitivity patterns to other antimicrobials. However, the MIC showed the emergence of strains with reduced sensitivity to ciprofloxacin (MIC90≥3 μg/ mL) and cefotaxime (MIC90≥2 μg/mL). Conclusion: The findings show high prevalence of nosocomial diseases-related bacterial species and low susceptibility to antimicrobial drugs. Therefore, these results imply caution against the indiscriminate use of broad spectrum antibiotics in dental practice.

Carcinoma epidemóide de labio inferior: estudo de 135 casos registrados no Hospital do Câncer de Pernambuco no período compreendido entre 1992 e 1997 / Epidermoid carcinoma of lower lip: study of 135 cases registred in Hospital do Câncer de Pernambuco in the period between 1992 and 1997

Os autores apresentam uma avaliaçäo de 135 prontuários de pacientes portadores de Carcinoma Epidermóide de Lábio Inferior atendidos no Hospital do Câncer de Pernambuco no período de 1992 a 1997. Os resultados mostraram uma predominância das lesöes em pacientes do sexo masculino (75,5 por cento); trabalhadores em exposiçäo solar contínua foram os mais acometidos (46,5 por cento); tabagistas (84 por cento); a faixa etária mais afetada foi acima dos 61 anos (69,5 por cento)