Human p100 protein:a novel coactivator that enhances ST AT6-mediated gene transcriptional activation / 中国免疫学杂志

Objective:To setup a stable cell line which can overexpress human p100 protein,and then st udy the physiologic functions of p100 in regulation of STAT6-mediated gene tran scription.Methods:Using coimmunoprecipitation method to identify protein-protein interactions.STA T6-mediated gene transcriptional activation was detected by luciferase assay.Results:Human p100 protein interacted with both STAT6 and RNA polymerase Ⅱ,resulting in the enhancement of STAT6-mediated gene transcriptional activation.Conclusion:Human p100 protein acts as a bridging factor between STAT6 and basal transcripti onal machenary. [