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A major issue in agriculture is the protection of crops against diseases and pests. Plant breeding has been primarily responsible for the growth of disease-resistant cultivars. The use of gene editing techniques in plant breeding is essential for obtaining desired features. Clustered Regular Interspaced Palindromic Repeats (CRISPER)/Cas9 (CRISPR-related protein) is a new advancement in gene editing technology. It can be utilised in plant defence mechanisms against pathogen attack by recognising the bacterial immune system and destroying invasive pathogen genes. Advances in plant breeding through CRISPR/Cas9 integration have helped develop cultivars including hereditary resistance to bacterial and viral diseases. Future crop generations can acquire CRISPR/Cas9-mediated transgene resistance if the Cas9/sgRNA transgene has been isolated in the F1 generation. Cas9/sgRNA transgene separation makes CRISPR/Cas9 safe for use in plant breeding. Although CRISPR/Cas9 has proven to be a wonderful tool to revolutionize plant breeding and develop various disease resistant varieties, its effect on many plant physiological processes remains to be thoroughly investigated.
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Laparoscopic cholecystectomy is one of the most common operations performed in general surgery. Elective laparoscopiccholecystectomy has a low risk for infective complications, and standard guidelines do not recommend prophylactic antibioticuse for low-risk cases. However, the use of antibiotic prophylaxis is very prevalent and the duration and dosage are inconsistentand varies widely among surgeons. This study is being done to assess the role of no antibiotic prophylaxis in the prevention ofwound infection in a patient undergoing elective laparoscopic cholecystectomy.Study Design and Period: The study was conducted in the Department of General Surgery at Christian Medical College,Ludhiana. This was an open labeled study conducted from the period of January 1, 2014, to December 31, 2014.Results: 92 patients suffering from chronic calculous cholecystitis undergoing elective laparoscopic cholecystectomy wereincluded in the study. Group A with 23 cases without prophylactic antibiotic. Group B with 69 cases with two doses of prophylacticantibiotics Inj. cefuroxime 1.5 gram 30 min prior to induction and after 6 h. The majority of our 81patients (88.04%) were females.The male to female ratio was 1:8. 3 patients (3.27%) in Group B had associated comorbidities except for diabetes mellitus. Themajority of patients 14 (60.86%) in Group A had taken 1-2 h of operative time while 58 patients (84.05%) patients in Group Bwith statistical insignificant P = 0.05. There was no fever in Group A patients while in Group B 2 patients (2.89%) had fever onsecond post-operative day which was not related to surgical site infection and that was due to superficial thrombophlebitis. InGroup A 18 patients (78.26%) were discharged on second postoperative days while 46 patients (66.67%) patients in Group Bwere discharged on second postoperative day. 8 patients (11.59%) in Group B were discharged on third postoperative days.There was no statistical difference in the duration of hospitalization between the two groups with P = 0.22. The overall incidenceof postoperative infective complications were nil in both groups either with patients having no prophylactic antibiotic or thosehaving prophylactic antibiotics
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In our study, we have compared inguinal hernia skin closure with octylcyanoacrylate tissue adhesive and subcuticular suturewith 4-0 Monocryl. We found that time require for closure is significantly less with using glue. Hospital stay are less in glue groupcompare to subcuticular group but not significant. Incidence of infection is more in subcuticular group. Cosmetic outcome of scaris equally good in glue as compared to sutures. Post op pain is less in glue group. Inguinal hernia is a common surgical problemand the usual treatment is Lichtenstein mesh hernioplasty. Wound closure is usually done by interrupted sutures, subcuticularsutures, or staples. Tissue adhesive octylcyanoacrylate is an alternative method for skin closure, which is a new generation longchain tissue adhesive. In this study, we will be comparing the outcome of tissue adhesive octylcyanoacrylate with subcuticularsuture for closure of inguinal hernia surgery in adults in terms of time efficiency, cosmesis, pain, and wound complications
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Antecedentes. La valoración del riesgo de violencia es un requisito fundamental en la toma de decisiones profesionales que implican prevenir, intervenir o informar sobre la conducta de las personas. El uso de herramientas estructuradas de evaluación del riesgo de violencia ha mostrado mejoría en la precisión de las evaluaciones basadas exclusivamente en el juicio clínico o en la pericia de un experto en contextos psiquiátricos, penitenciarios y jurídicos. Objetivo. Este estudio presenta los resultados de la primera encuesta sobre las prácticas profesionales asociadas al uso de herramientas de evaluación del riesgo de violencia en España. Materiales y métodos. La información fue recogida mediante la administración de una encuesta en internet que fue distribuida por correo electrónico a los miembros de organizaciones profesionales. Resultados. De manera similar a los contextos profesionales del resto del mundo, las escalas de psicopatía de Robert Hare (Psychopathy Checklist-Revised y Psychopathy Checklist: Screening Version) y el Historical-Clinical-Risk Management-20 encabezaron la lista de las herramientas más usadas tanto por elección personal como por requisito institucional. Conclusiones. Se ofrecen datos novedosos sobre la prevalencia de uso y la utilidad percibida de las herramientas estructuradas, así como sobre otras cuestiones relacionadas a las prácticas profesionales de evaluación del riesgo de violencia en España que pueden orientar tanto a los profesionales de contextos sanitarios, correccionales y forenses, como a los responsables de las instituciones en la elección de las herramientas a implementar para asistirlos en la toma de decisiones.
Background. Violence risk assessment is a key requirement in professional decision making involving prevention, intervention or reporting on human behavior. The use of structured tools for violence risk assessment has shown to improve the accuracy of assessments based exclusively on clinical judgment or expertise in psychiatric, correctional and legal settings. Objectives. This study presents results of the first survey about professional practices associated with tools for violence risk assessment in Spain. Materials and methods. The information was collected by administering an online-based survey that was distributed by e-mail to members of professional organizations around the country. Results. As in professional contexts worldwide, the Robert Hare's psychopathy scales (Psychopathy Checklist-Revised and Psychopathy Checklist: Screening Version) and the Historical-Clinical-Risk Management-20 topped the list of the most used tools both by professional choice and institutional requirement. Conclusions. We provide novel data on the prevalence of use and the perceived utility of specific tools, as well as on other issues related to the professional practice of violence risk assessment in Spain, which can guide professional in the health care, correctional and forensic settings, as well as those responsible for decisions in institutions about choosing which tool to implement.
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Introducción: En las últimas décadas hubo importantes avances científicos en la sistematización de la evaluación del riesgo de violencia, pero esas contribuciones no se implementan de manera rápida ni uniforme en la práctica latinoamericana. Objetivos: Describir qué instrumentos de evaluación de riesgo de violencia se utilizan en Chile y Argentina, y qué características tiene esa práctica profesional. Material y método: Se realizó una encuesta a través de la web, a profesionales relacionados con la salud mental registrados en listas nacionales e internacionales de 17 países, entre septiembre y diciembre del 2012. En el estudio se informan resultados de 46 respondientes de Chile y Argentina. Resultados: El 78% de los psiquiatras, el 93% de los psicólogos y el 100% de los trabajadores sociales utilizaron instrumentos para ERV; la amplia mayoría los consideró relativamente útiles. Los instrumentos más utilizados fueron HCR-20, PCL-R y PCL-SV.
Introdução: Nas últimas décadas houve avanços científicos importantes na sistematização da avaliação do risco da violência, mas aquelas contribuições não são executadas rapidamente nem uniformemente na prática latino-americana. Objetivos: Descrever que instrumentos da avaliação do risco da violência são usados no Chile e na Argentina, e que características tem essa prática profissional. Material e método: Uma pesquisa foi feita através da Web, aos profissionais relacionados à saúde mental registrados nas listas nacionais e internacionais de 17 países, entre setembro e dezembro do 2012. No estudo os resultados de 46 respondentes do Chile e da Argentina são informados. Resultados: 78% dos psiquiatra, 93% dos psicólogos e o 100% dos trabalhadores sociais usaram instrumentos para ERV; a ampla maioria os considerou relativamente úteis. Os instrumentos mais usados foram HCR-20, PCL-R e PCL-SV.
Introduction: The last decades revealed important scientific advances in the systemization of violence risk assessment; nevertheless, the implementation in practice of these contributions have neither been fast nor uniform in Latin America. Objectives: The article is aimed at describing the instruments for violence risk assessment used in Chile and Argentina and the features of this professional practice. Material and method: A web survey was carried out among mental health-related professionals registered in the national and international lists of 17 countries between September and December 2012. The results obtained from 46 respondents from Chile and Argentina are reported in this study: 78% of the psychiatrists, 93% of the psychologists and 100% of the social workers involved have employed violence risk assessment (VRA) instruments deemed relatively useful by a wide majority, being HCR-20, PCL-R and PCL-SV the most commonly used.
Sujet(s)
Psychiatrie légale/instrumentation , Psychiatrie légale/méthodes , Psychiatrie légale/tendancesRÉSUMÉ
Background. Systematic reviews and metaanalysis often come to conflicting conclusions on key issues and have a number of potentially important methodological limitations. A metareview represents one approach to a descriptive investigation of such issues in review literatures; this involves a systematic review of previously published reviews. Metareviews report on the areas that systematic reviews and metaanalyses have covered, investigating the methodological quality of such reviews, comparing methods for reporting results with recommended standards in the field of systematic reviewing and highlighting areas which could benefit from further research. Objective.The present report was aimed at critically examining the reporting quality of available medical metareviews and encouraging the use of such innovative approach to develop an instrument for assessing metareviews' methodological quality. Methods. PsycINFO, MEDLINE, EMBASE, and CINAHL were searched for previous medical metareviews as of February 11th, 2012. References regarding identified reports and annotated bibliographies were used to supplement the search. Results. Four metareviews meeting the inclusion criteria were identified and descriptively analysed. The first set of standardised metareview reporting guidelines' checklist (metareview assessment of reporting quality MARQ), using quality checklists developed for primary studies and reviews as models, was introduced to enable transparent and consistent reporting of metareview methodology. An average of 15 (SD = 3) MARQ criteria were met when applied to the four metareviews identified during the systematic search. This indicated a moderate level of reporting quality which should be improved in subsequent applications of the methodology by using the standardised checklist. A high level of interrater agreement was found (κ = 0.93). Conclusion. The standardised set of guidelines outlined in this report should assist future researchers in conducting more transparent and methodologically rigorous metareviews.
Antecedentes. Las revisiones sistemáticas y metaanálisis a menudo llegan a conclusiones contradictorias sobre cuestiones fundamentales y tienen una serie de limitaciones metodológicas potencialmente importantes. Una metarevisión representa una aproximación a una investigación descriptiva de estos temas en la literatura de revisión, lo que implica una revisión sistemática de las revisiones publicadas anteriormente. Las metarevisiones informan sobre las áreas que las revisiones sistemáticas y metaanálisis han cubierto, investigando la calidad metodológica de dichos estudios, y comparando los métodos para informar resultados con las normas recomendadas en el campo de las revisiones sistemáticas y poner de relieve áreas que se podrían beneficiar de futuras investigaciones. Objetivo. Examinar críticamente la calidad de la información disponible de las metarevisiones médicas y fomentar el uso de tal enfoque innovador para desarrollar un instrumento que evalúe la calidad metodológica de las metarevisiones. Métodos. En las bases de datos PsycINFO, MEDLINE, EMBASE, y CINAHL se buscaron metarevisiones médicas hasta el 11 de febrero de 2012. Para complementar la búsqueda fueron usadas referencias relativas a los informes identificados y las bibliografías anotadas. Resultados. Cuatro metarevisiones cumplieron los criterios de inclusión las cuales fueron identificadas y analizadas de forma descriptiva. El primer conjunto de directrices de la lista de verificación estandarizados metareview (Lista de verificación de la Evaluación de los informes de calidad de las Metarevisiones (MARQ)), uso listas de control de calidad desarrollados por los estudios primarios y revisiones como modelos, se introdujo para permitir un registro transparente y coherente de la metodología de las metarevisiones. Un promedio de 15 (SD=3) MARQ criterios se cumplieron cuando se aplicaron a las cuatro metarevisiones identificadas durante la búsqueda sistemática. Esto indica un nivel moderado de calidad de los informes que deben ser mejorados en las aplicaciones posteriores de la metodología utilizando la lista de verificación estandarizada. Se encontró un alto nivel de acuerdo interevaluadores (κ=0,93). Conclusión. El conjunto estandarizado de directrices que se describen en este informe debería ayudar a los futuros investigadores la realización de metarevisiones más transparentes y rigurosas metodológicamente.
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Discharge from the ear is one of the commonest symptoms of infections of the ear. To study the major strains of bacteria encountered in ear discharges, this study was conducted from July 1999, to June 2001 in the microbiology laboratory of Om Hospital. A total of 115 patients, of 6-35 years of age clinically diagnosed as discharging otitis media were studied. In 60.9% single organism and in 26.08% two organisms were isolated. Results revealed that, Staphylococcus aureus (34.3%), Klebsiella pneumoniae (24.3%), Pseudomons aeruginosa (22.8%), Enterobacter sps (7.1%), Esch coli (4.3%), Citrobacter sps (1.4%), Proteus sps (1.4%), Acinetobacter sps (1.4%) and Pneumococci (1.4%) were the common organisms in the discharge. Candida albicans (2.8%) was the pathogen in two cases. In 27.8% of cases the causative agent was not demonstrated. About sixty percent (65.9%), 64.8% and 56.0% of the isolates were sensitive to amikacin, ciprofloxacin and gentamycin respectively.