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The Present study was conducted to assess the groundwater inventory in Jamrani Dam Command situated in Uttrakhand and Uttar Pradesh, India. Groundwater inventory was prepared for different blocks of Udhamsingh Nagar, Rampur, and Bareilly districts in Jamrani Dam Command from 1988 to 2010 at an interval of five years. Over-exploitation of groundwater has created an alarming situation in a few blocks of Rampur and Bareilly districts in Jamrani Dam Command. In the year 2010, the Bilaspur, Baheri, Gadarpur, and Rudrapur blocks of the study area were found under the safe category; Swar and Meerganj blocks were found under the critical category whereas Milak and Shergarh blocks were found under the over-exploited category. The maximum and minimum stages of development were found at 109.08% and 59.31% at Shergarh and Rudrarpur blocks, respectively. The overall stage of development for the study area was 68.44% and the area as a whole may be put under the semi-critical category for the year 2010. In the present study, the physical-chemical properties of groundwater in shallow aquifers and their suitability for drinking, irrigation, and industrial uses were studied. The physical-chemical properties of the groundwater of the study area were determined using standard methods of water quality analysis. A comprehensive and proper groundwater recharge plan for the identified landforms with their prevailing geomorphic features in the study area has been suggested to maintain the groundwater at a safe and desired level in the future in the Jamrani Dam Command. Various water recharging structures namely: a series of check dams, percolation tanks, farm ponds and nallah bunds are most possible techniques for recharging groundwater aquifers by utilizing excess water available during monsoon season in the form of runoff obtained from the land surface. Geographic information Systems (GIS) and Remote Sensing were applied to the study area to delineate different land uses from the satellite imageries (IRS-1D). The study area delineated from the S.O.I. toposheets was also classified into nine land use classes i.e. agricultural land, grassland, water body, scrubland, and forest etc.
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Background: Diabetes mellitus and hearing loss are common public health problems. Sensorineural hearing loss in Type 2 diabetes mellitus patients is an underestimated complication. The aim was to study the correlation between level of HbA1c and degree of sensorineural hearing loss. Methods: It was a cross sectional study carried on 130 known cases of T2DM, aged 25-50 years. Various confounding factors such as coexisting hypertension, ear disease, ototoxic drug intake, any neuropathic disorder was excluded in advance. All patients underwent clinical ear examination and pure tone audiometry test. Their level of HbA1c was measured. This value of HbA1c was correlated with the hearing threshold. Results: Out of 130, 58 subjects had normal hearing while 72 had hearing impairment. Among subjects (72 out of 130) having hearing impairment, significant correlation (p-value 0.035 for right ear and 0.029 for left ear) between hearing threshold and HbA1c was found. Also, level of HbA1c significantly (p-value 0.049) correlated with degree of hearing loss. Conclusions: Hearing loss is an underestimated complication of T2DM. This needs to be addressed with periodic hearing assessment of diabetes patients.
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Background: The gold standard technique for preserving a patent airway throughout anaesthesia is tracheal intubation. I-gel is a relatively new addition to the SADs. I-gel has combined the concept of the non-cuffed SADs like the SLIPA and gastric tube of the proseal LMA yet retaining the shape of laryngeal mask. Hence, we have compared ease of insertion, number and duration of insertion attempts among the two devices. Methods: This study was conducted on patients undergoing elective surgery under GA in Pacific Medical College and Hospital, Udaipur. Patients were divided into two groups: group A= LMA classic, a variant of supraglottic airway device and group B= I-gel, a variant of supraglottic airway device. The two devices were than compared with respect to success rate of insertion, time taken for insertion and ease of gastric tube placement (number of attempts) and post-operative airway morbidity. Results: Mean insertion time was 8.66±3.21 seconds in C-LMA and 6.49±1.92 seconds in I-gel (p<0.001). 97.14% was the success rate of single time attempt insertion with I-gel as compared to 88.57% in C-LMA. There was a failure rate of 11.43% in single attempt insertion with CLMA as compared to 2.86% with I-gel (p>0.05). Conclusions: We hereby concluded with our study that successful and shorter duration of insertion, with less hemodynamic response, makes I-gel™ a suitable alternative to LMA classic™ during general anesthesia.
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A stroke, or brain attack, happens when blood flow to the brain is stopped. It is an emergency situation. The brain needs a constant supply of oxygen and nutrients in order to work well. If blood supply is stopped even for a short time, this can cause problems. Brain cells begin to die after just a few minutes without blood or oxygen. Stroke is the second most common cause of death worldwide and the most important cause of physical weakness, with a growing occurrence in developing countries. Ischemic stroke is the most common type of stroke which occurs due to arterial block and managed by fast reperfusion with endovascular thrombectomy and intravenous thrombolysis. The first step in stroke management is initial identification of patients with stroke and triage to centers accomplished of transporting the suitable treatment, as fast as conceivable. Mobile stroke units, tele stroke and artificial intelligence are technologies playing an important role in recognizing and treating stroke. Stroke system-of-care models remain to streamline the distribution of definitive revascularization in the age of mechanical thrombectomy. Overall, in the present review, we emphasized the pathophysiology, diagnosis and management of acute ischemic stroke (AIS).
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A kidney transplant, sometimes known as a renal transplant, is the treatment of choice for kidney failure at end stage renal disease (ESRD). The renal transplant surgery is followed by a lifetime course of immunosuppressive agents, divided into initial induction phase and later maintenance phase. It is seen that the risk of acute rejection is maximum in the initial months after transplantation (induction phase) and then reduces later (maintenance phase). In induction phase there is use of high-intensity immunosuppression immediately after transplantation, when the risk of rejection is maximum and then the dose reduced for long- term therapy. The main challenge in the renal transplantation community is long- term transplant survival. Long-term graft loss is mainly due to acute and chronic graft rejection, and also due to complications of immunosuppressive therapy. Currently, there is triple therapy as conventional immunosuppressive protocol: a calcineurin inhibitor, an antimetabolite agent, and a corticosteroid. The main aim of development of new immunosuppressive agents is not only improvement of short- term outcomes but also to increase the long- term graft survival by less nephrotoxicity, and minimal side-effects.
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Background: Adverse drug reactions (ADRs) are one of the prime causes of morbidity and mortality, increase in hospital stay and socioeconomic burden on the patients. Periodic monitoring aids in formulating methods for safe usage of medicines in hospitals. Identification of ADRs and their reporting pattern can provide useful information for their prevention. Hence this study was done to see the pattern of reported ADRs in Patna Medical College and Hospital, Patna in a 3 months of study., Methods: It was an observational and retrospective study carried out between July 2022 to September 2022. Both outpatients and inpatients were included in the study. The ADRs in the form of Individual Case Safety Reports (ICSRs) were sent to the nearby adverse drug reaction monitoring centre (AMC)., Results: The occurrence of ADRs was more common in females (56.25%) as compared to males (43.75%). Patients of age-group 21-40 years (40.625%) were most commonly involved. Medicine department (34.375%) reported the maximum percentage of ADRs. Antimicrobials (37.50%) was the most common drug-group causing ADRs. Maximum reported ADRs (81.25%) were probable, 9.375% were possible, 6.25% were certain, while 3.125% were unlikely with the suspected drug as per Naranjo scale., Conclusions: The pattern of ADRs reported in our hospital is comparable with the results of studies conducted in hospital setup elsewhere, along with a few differences. The study results revealed opportunities for interventions in ADR management especially for the preventable ADRs to ensure safer drug use.
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Aim: The study aimed to evaluate the effects of hydrogel application on growth, physiological responses and yield of capsicum under high altitude cold desert conditions with deficit irrigation of Ladakh region. Methodology: Field experiment was conducted with five levels of hydrogel treatment (viz. 0, 5, 10, 15 and 20 kg ha-1) at cold desert Ladakh region with two irrigation intervals (7 and 11 days) during 2014-15 cropping season. Plant growth characters, root morphology, physiological parameters and capsicum yield were analyzed according to standard methods during the experiments. Results: Hydrogel application in deficit irrigation improved physiological parameters viz. leaf chlorophyll content, relative water content, membrane stability index and significant reduction in electrolyte leakage in capsicum plant. Significant improvement in root morphology was also recorded when hydrogel was applied @ 15 and 20 kg ha-1. Improvement of yield attributing characters as influenced by hydrogel application @ 15 and 20 kg ha-1 resulted in significant increase in capsicum yield with reduced water requirement. Interpretation: Hydrogel has potential for growth of capsicum crop through improved root morphology and physiology of plant in deficit irrigation under arid and semi-arid regions where availability of water for irrigation is scarce or average rainfall is quite low.
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Background: The gastrointestinal tract of the HIV/AIDS patient (s) is harboured by a number of luminal extracellular and intracellular parasites. These parasites are responsible for deteriorating and worsening the general health condition(s) of these immunocompromised groups of patients. Moreover, these parasitic infections are often correlated with the CD4 counts of the individuals. Therefore, with a view to assess the relationship between the presence of parasitic infection and that of the CD4 count, the present work is being undertaken.Methods: The study was conducted in the Parasitology section of the department of Life Sciences, Manipur University and CD4 count unit of Microbiology Department, RIMS hospital Imphal from 34 HIV positive Antiretroviral treatment (ART) naive patients, aged between 19 to 50 years. The samples were taken from the patients only after obtaining a signed consent form . Techniques like normal saline method, iodine wet preparation method, formol ethyl acetate concentration technique, Baermann modified funnel technique, modified Ziehl Neelsen staining technique and adhesive cellophane tape method were employed for the present investigation.Results: The present study reveals that patients with CD4 count <200 cells/μl were harbouring only two parasites (Cryptosporidium sp and G. lamblia) whereas individuals with CD4 count within the range of 200-500 cells/μl were observed to have harboured G. lamblia, Cryptosporidium sp, E.histolytica, hookworm, S. stercoralis and A. lumbricoides. On the other hand, enteric parasites associated with CD4 count >500 cells/μl included G. lamblia, hookworm, E. vermicularis and S. stercoralis.Conclusions: The present study shows that occurrence of intestinal parasitism is independent of CD4 count in HIV infected patients. Therefore, based on the present findings, it may be suggested that irrespective of CD4 count(s) and whether the patient experiences diarrhoea or not, regular monitoring of the HIV patient(s) for the presence of enteric parasitosis by adopting standard laboratory protocols should be encouraged for maintaining a healthy life of the people living with HIV/AIDS.
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Background and Objective: Japanese encephalitis (JE) surveillance is not well established in many countries, and laboratory confirmation is challenging, the true extent and prevalence of the virus and burden of disease are not well understood. It is estimated that 67,900 clinical cases of JE occur annually despite the widespread availability of vaccine, with approximately 13,600–20,400 deaths and an overall incidence rate of 1.8/100,000 in the 24 countries with JE risk. The present study aimed at determining the prevalence rate (PR) and distribution (time, place and person) of JE cases in Manipur. This descriptive study was conducted over 24-month period (2016–2017). Materials and Methods: A total of 1770 cases of acute encephalitis syndrome tested for JE including 251 confirmed JE were diagnosed by IgM antibody-capture enzyme-linked immunosorbent assay. Results: The JE cases were most commonly reported in the age group of >15 years. Most of JE prevalence was seen in rural distribution in our study. There is a strong seasonal pattern of JE occurrence in Manipur which peaked in July–August and declined by October each year, which corresponds to the monsoon season. The JE cases were reported in all the districts of the state expanding in the plains and hill regions. Conclusions: The changing pattern of JE cases among different age groups was also observed in our study. The present study reveals the changing pattern of the prevalence of JE in the State of Manipur and initiated a systematic approach of JE surveillance also highlights the need for further expanding of surveillance across the state.
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Background: We report an assessment of measles outbreak during the months of February 2014 to April 2014 in Dwarahat block of district Almora and the response mounted to it. Materials and Methods: An intensive door‑to‑door search to six measles affected villages in Dwarahat block of district Almora, covering a population of 2,408 was carried out to identify the cases of measles by a rapid response team (RRT). A total of ten blood samples were randomly collected for detecting IgM antibody against measles. For all cases, information on personal details, place of residence, time of onset and status of immunization were obtained. Results: Overall attack rate (AR) was 2.8%. AR among the population of age‑group 0–16 was 7.2%. Statistically significant higher AR (16.26%) was seen for the age‑group of 0–5 years as compare to 6–10 and 11–16 years of age (AR‑8.71, relative risk‑0.53, 95% confidence interval‑0.32–0.88, P value‑0.012 and AR‑0.57%, relative risk‑0.035, 95% confidence interval‑0.00–0.14, P value‑0.000, respectively). Males were affected more often than females 35 [59.2%] vs. 24 [40.8%]. Measles‑related complications were seen in three children. No death was reported. Of the 10 samples, nine were positive for measles IgM antibodies by enzyme‑linked immunosorbent assay (ELISA). Conclusion: The recognition of early warning signals, timely investigation and application of specific control measures can contain the outbreak. The unvaccinated or partially protected human beings serve as the reservoir of measles virus. Hence, there is a need for sero surveillance for measles in Uttarakhand and one catch up measles immunisation campaign to prevent future outbreak.
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Alveolar ridge atrophy poses a clinical challenge towards the fabrication of a successful prosthesis. Resorption of denture bearing areas results in an unstable non-retentive dentures associated with pain and discomfort. Prosthetic Rehabilitation of a patient with severely resorbed ridge is the most challenging therapy a prosthodontist can undertake. For a favourable prognosis of the denture therapy, impression technique selected should be based on the present state of the basal tissue support. This article presents rehabilitation of a patient with severely resorbed mandibular ridge with the application of neutral zone concept being incorporated into impression making in an effort to achieve successful complete denture therapy. Finally, patient had more stable, retentive and functionally efficient complete denture. In conclusion, neutral zone impression technique proved to be a quite effective method for prosthetic rehabilitation of patient with severe mandibular ridge resorption.
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Background: Pregnancy induced hypertension is a common medical complication associated with pregnancy. When untreated ,it has serious implications on maternal and fetal health. Labetalol is a new an anti hypertensive agent with alpha adrenergic and non selective beta –adrenergic receptor blocking actions. Objectives: 1)To assess the effect of labetalol on control of blood pressure in moderate to severe Pregnancy induced hypertension2)To study the side effects of labetalol 3)To analyze the maternal and perinatal outcome. Material and Methods: A prospective observational study of 50 cases of moderate to severe Pregnancy induced hypertension, treated with Labetalol ,was conducted over a period of two years. Cases were divided in Labetalol-respondent and non respondent group. Pregnancies from non-respondent group were terminated either by induction of labour or by caesarean section .Cases who responded to labetalol, were treated with oral Labetalol. Obstetric and perinatal outcome in both the groups was analyzed. Results: Fifty six percent cases did not respond to intravenous labetalol therapy (maximum dose 120 mg over 30 minutes) and thus pregnancy was terminated. Forty four percent cases responded to labetalol therapy and pregnancy was continued for average of 8 days .Forty eight percent cases were delivered by caesarean section. Seventy eight percent babies were live born .There were twelve stillbirths and five neonatal deaths .Twenty percent cases had mild and transient side effects related to labetalol. Conclusion:The effective control of blood pressure with the use of Labetalol in the dosage used ,was observed in less than fifty percent cases of moderate to severe pregnancy induced hypertension.
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Nematode trapping fungi captured nematode by forming different types of structure in a way to get supplement of nitrogen from the nematode, just like carnivorous plants. Fungal parasites of nematodes are broadly classified as either predaceous or endoparasitic. The genus Nematoctonus is unique in that some species are endoparasitic and some species are predaceous by established criteria. The generic diagnosis for Nematoctonus was given by Dreschsler that one assimilative hyphae which is hyaline, more or less branched, developing within minute living animals. Growth and sporulation of N.robustus was significantly varied with different temperature on corn meal agar medium. It is evident from results showing the effect of temperature on the biology of N.robustus that 200C was most optimum temperature for both growth and sporulation. With increase in the temperature there is increase in radial growth of this fungus but correspondingly there s decrease in sporulation.
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The plant parasitic nematodes infect the root tissues of the plant causing root galls that lead to reduced water and mineral uptake in the plant root system. Nematophagous fungus are used as biocontrol for the nematodes. Among those Nematoctonus are one of the species used as bioagent. Nematoctonus species produces an extensive mycelium and capture many nematodes with hour glass shaped adhesive knobs on the hyphae. Nematodes become attached to these adhesive knobs and the cuticle of nematode is penetrated by the infective hyphae. This isolate of Nematoctonus robustus is characterized by hyaline mycelium, dikaryotic in nature containing genetically two different nuclei in each cell, having distinct clamp connection. The fungus has better colonizing ability on natural solid substrates like wheat straw and rice straw. It also show good ability to colonize on different cereal grains and various other waste products like coconut coir and FYM etc. This species is one of the best used for mass production and effective for control of plant parasitic nematodes.
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Background: Urolithiasis is the third most common urological disease. The prevalence is on the rise due to various changes in the socio-demographic and other etiological factors in the north-eastern states of India in general and Manipur in particular. Aims & Objective: The study was conducted to determine the prevalence of urinary stones and to assess the association between urolithiasis and selected variables of interest. Material and Methods: The study was conducted during September 2008 to August 2010 among wetland dwellers of Loktak Lake in Thanga, a rural area of Manipur. A representative sample of subjects, which included men and women 15 years of age and above were included in the study. Sample size was calculated based on a prevalence rate of 5.41% with an allowable error of 1.5 at 95% confidence level. Calculated sample size was 875. A pre-tested interview schedule was used for data collection. Results: A total of 875 individuals were studied. The prevalence rate of urolithiasis was 196(22.40%) and was more commonly present in the age group of 25-44 years. Urolithiasis was observed to be significantly more common among those individuals who are more educated, engaged in heavy work, with history of urinary tract infection (UTI), with history of stressful events in recent years, among those who consumed only 2-4 glasses of water per day and with family history of stones. Conclusion: Prevalence of urolithiasis was observed to be 196(22.4%). Information regarding the risk factors which have been identified in the present study can be utilized in formulating future health plans for preventive services.
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Meckel-Gruber syndrome, also known as ‘Dysencephalia splanchnocystica’, is a rare lethal autosomal recessive disorder consisting of central nervous system malformation- mainly posterior encephalocele (80%), multicystic kidneys (95%) and polydactyly (75%). Besides the classic triad of neural tube defects, polydactyly and cystic dysplasia of the kidneys, other abnormalities can occur in association with the syndrome, which may be detected sonographically include micrognathia, cardiac abnormalities, syndactyly, clinodactyly and clubbed foot. We report a case of a 26-year-old woman with previous LSCS referred from a private practitioner with abnormal ultrasonographic findings. She was diagnosed to have Meckel-Gruber syndrome. Woman and her husband were counseled regarding this lethal condition incompatible with life and after proper consent and information, pregnancy was terminated.
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Mesenchymal stromal cells (MSC) are multipotent cells that can be derived from many different organs and tissues. While there are many ways to label and track cells each with strengths and weakness, the green fluorescent protein (GFP) is a reporter gene commonly employed. In the present study, caprine MSC were collected from bone marrow and cells were characterised with MSC specific markers. Passage 10 (P10) MSC cells were transfected using plasmid vector containing GFP as reporter gene with different concentrations of DNA and lipofectamine. Six different concentrations of DNA and lipofectamine as 1 µg DNA: 2 µL lipofectamine, 1 µg DNA: 2.5 µL lipofectamine, 1.2 µg DNA: 2.2 µL lipofectamine, 1.2 µg DNA: 2.5 µL lipofectamine, 1.5 µg DNA: 2.5 µL lipofectamine, 1.5 µg DNA: 3 µL lipofectamine were used. After 24 h and 48 h of transfection, caprine MSC were observed under florescent microscope. Highest transfection rate indicating green flourecscent MSC were found when the cells were transfected with 1.2 µg DNA: 2.2 µL lipofectamine and 1.5 µg DNA: 2.5 µL lipofectamine than other combinations. These cells have been propagated beyond 4th passage maintaining GFP expression. The results indicated that stable GFP positive MSC cells can be generated using the above protocol. These cells are being used for transplantation studies.
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Animaux , Marqueurs biologiques/analyse , Cellules cultivées , Vecteurs génétiques , Capra , Protéines à fluorescence verte/génétique , Protéines à fluorescence verte/métabolisme , Caryotypage , Lipides , Cellules souches mésenchymateuses/cytologie , Cellules souches mésenchymateuses/métabolisme , ARN messager/génétique , Réaction de polymérisation en chaine en temps réel , RT-PCR , TransfectionRÉSUMÉ
Background. We prospectively studied the prevalence, type and causes of anaemia in newly diagnosed patients with lymphoid malignancies. Methods. Between January 2007 and June 2008, a total of 316 newly diagnosed, consecutive patients (aged 15 years or above) of Hodgkin lymphoma, non-Hodgkin lymphoma and chronic lymphocytic leukaemia with anaemia (haemoglobin <11 g/dl), were analysed to determine the prevalence and a subgroup of 46 patients was analysed for the cause of anaemia. Results. Hodgkin lymphoma, non-Hodgkin lymphoma and chronic lymphocytic leukaemia were the diagnoses in 81 (25.8%), 203 (64.7%) and 30 (9.6%) patients, respectively. Anaemia was present in 134 patients (42.4%). Anaemia of chronic disease was present in 33/46 (71.7%) and iron deficiency in 18/46 (39.1%) patients. Vitamin B12 and/or folate deficiency was detected in 10/46 (21.7%) patients (B12 deficiency alone in 7, folate deficiency alone in 1 and combined B12 and folate deficiency in 2). Autoimmune haemolytic anaemia was detected in 5/46 (10.9%) although direct Coombs test was positive in 17/46 (37%) patients. Among patients with Hodgkin lymphoma and non-Hodgkin lymphoma, anaemia due to bone marrow involvement was present in 16/40 (40%). In most patients with bone marrow involvement, anaemia was due to other causes. In only 3 patients, anaemia was attributable to bone marrow involvement alone. Anaemia was multifactorial in 18/46 (39.1%) patients. Nutritional deficiency alone or in combination was present in 22/46 (47.8%) patients. Conclusion. Anaemia is common in lymphoid malignancies at initial presentation. Besides managing anaemia of chronic disease and bone marrow involvement, nutritional and autoimmune causes should be ruled out.
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Adolescent , Adulte , Anémie/épidémiologie , Anémie/étiologie , Anémie hémolytique auto-immune/épidémiologie , Anémie hémolytique auto-immune/étiologie , Anémie par carence en fer/épidémiologie , Moelle osseuse/anatomopathologie , Femelle , Carence en acide folique/complications , Maladie de Hodgkin/complications , Humains , Leucémie chronique lymphocytaire à cellules B/complications , Lymphome malin non hodgkinien/complications , Mâle , Adulte d'âge moyen , Prévalence , Études prospectives , Carence en vitamine B12/complications , Jeune adulteRÉSUMÉ
Background & objectives: Phlebotomus argentipes is the vector for visceral leishmaniasis in India. The development of resistance in kala-azar vector to DDT has been reported from various parts of India. The main objective of this study was to generate information on insecticides susceptibility status of P. argentipes to DDT, malathion and deltamethrin in different parts in three states of India. Methods: Phlebotomus argentipes were collected from different villages, identified and used to investigate the susceptibility status against DDT, malathion and deltamethrin as per the WHO standard methods. Results: Phlebotomus argentipes was resistant to DDT in different areas, viz. PHCs Murumgaon in Maharashtra; Ramgarh in Jharkhand; Kodah, Falka, Mahua and Lalganj in Bihar. In Phulwari Shareef PHC of Patna district in Bihar, DDT produced 89% mortality in P. argentipes, indicating resistant/tolerance (verification required) to DDT. The corrected percent mortality to malathion (5%) in different areas ranged between 98 and 100%; and to deltamethrin (0.05%) between 98.4 and 100%. The results showed that the tested P. argentipes are susceptible to malathion and deltamethrin. Conclusion: Phlebotomus argentipes are still susceptible to malathion and deltamethrin, but resistant to DDT. The susceptibility status of P. argentipes should be monitored regularly in diversified situations to ascertain the judicious use of insecticides being used for indoor residual spraying in the programme for rational use of appropriate insecticide.