Botanical study of Meryta denhamii seem and its anthelmintic activity against Fasciola gigantica

The anthelmintic activity of the ethanolic extracts of stems and leaves of Meryta denhamii Seem, against adult liver flukes [Fasciola gigantica] was studied in vitro. Although leaves extract was inactive, stems extract exhibited anthelmintic activity and recorded LC[50] and LC[90] values, 16 and 26 gm/1 respectively. The mode of action of the ethanolic extract of the stems on the adult flukes was evaluated by scanning electron microscopy [SEM]. Tegumental sloughing, loss of spines and deformity of suckers were observed. These damages are responsible for the vermicidal effect of the ethanolic extract of the stems. The saponin contents of the stems [4.25%] and leaves [2.45%] were determined using haemolytic index. The plant was identified based on the macro and micromorphological features of the stem and leaf

Molluscicidal saponins from leaves of Hedera canariensis

Activity-guided fractionation of the methanol extract of Hedera canariensis [var. Gloire de Maringo] Wild leaves afforded five saponins 1-5. Chemical and spectral methods [MS, [1]HNMR, [13]CNMR] showed that they are glycosides of hederagenin and oleanolic acid. The results showed that 4,5 exhibited molluscicidal properties, compound 1 was inactive. Mortality rate of exposed snails increased by increasing plant extract concentration. Lymnaea cailliaudi was more sensitive to plant extract than Biomphalaria alexandrina. The histopathological study revealed distinct damage in the structure of the stomach and ovotestis of treated L. cailliaudi snails. Saponin content was determined in term of haemolytic index