RÉSUMÉ
Objective To explore the role of IEGs in the central neurotoxicity of mice induced by anatoxin-a ANTX-A. Methods 80 Kunming mice were divided into groups and treated with ANTX-A at 200, 50 and 12.5 ?g/kg respectively i.p. for one month, 0.9% sodium chloride solution was used as the control. The expression of c-fos and NGFI-A genes and CREB protein in mouse brain were determined by using RT-PCR and immunocytochemical methods. Results The expression of c-fos gene significantly increased in the females of 50, 200 ?g/kg groups and in the males of 200 ?g/kg group compared with the control group. The expression of C-FOS and CREB-1 protein was significantly higher in 200 ?g/kg group than that in the control group. In all ANTX-A treated groups, the expression of NGFI-A gene did not show a significant difference compared with the control. Conclusion c-fos and CREB-1 genes may play a role in the central neurotoxicity induced by anatoxin-a.
RÉSUMÉ
Objective To study the genotoxicity of bromate,a chief by-product of ozonated drinking water.Methods Genotoxicity at gene,DNA and chromosome levels induced by bromate DBP was determined using Ames test,unscheduled DNA synthesis(UDS)assay and micronucleus test(MNT)of mice bone marrow polychromatic erythrocytes respectively.Results In Ames test,bromate showed no mutagenic effect on salmonella typhimurium TA98and TA100strains with or without S9(rat liv-er metabolic activation system)compared with the negative control group,but the results of UDS assay showed that bromate could induce unscheduled DNA synthesis in rat primary hepatocytes and the results of MNT indicated that bromate increased the micronucleus rate of bone marrow polychromatic erythrocytes of mice.Conclusion It was suggested that bromate DBP might have genotoxicity at DNA and chromosome levels.