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1.
The Journal of the Korean Academy of Periodontology ; : 135-142, 2008.
Article Dans Coréen | WPRIM | ID: wpr-209386

Résumé

PURPOSE: Numerous studies have shown that crestal bone resorption around the implant was related to the location of the implant abutment junction(IAJ). Recently it was hypothesized that platform switching termed the inward horizontal repositioning of the IAJ might limit bone resorption around the implants. The purpose of this clinical study was to evaluate the effect of platform switching on crestal bone resorption. MATERIALS AND METHODS: The crestal bone loss of 65 external hex implants in 26 patients were radiographically measured at crown placement and follow-up examinations. 23 standard implants(non-platform switching group, NP) were connected with the matching abutments and 42 wide implants(platform switching group, PS) were connected with the 1 mm smaller diameter abutments. RESULTS: There was significant difference of crestal bone loss between NP group and PS group. For implants in the NP group, mean crestal bone loss was 1.18+/-0.68 mm at crown placement and 1.42+/-0.41 mm at follow-up. The meal bone loss in PS group was 0.47+/-0.52 mm at crown placement and 0.60+/-0.65 mm at follow-up. When the crestal bone changes according to placement depths of implants were compared, subcrestal position of IAJ had a significantly less bone loss in PS group, but it was not in NP group. CONCLUSION: Within the limits of the present study, it was concluded that platform switching technique might decrease crestal bone loss around the implants. Additionally, when the IAJ of implant was placed 1 mm deeper in the alveolar bone, the effect of platform switching on bone loss was enhanced.


Sujets)
Humains , Résorption osseuse , Couronnes , Implants dentaires , Études de suivi , Repas
2.
The Journal of the Korean Academy of Periodontology ; : 247-252, 2008.
Article Dans Coréen | WPRIM | ID: wpr-189253

Résumé

PURPOSE: Passive eruption is characterized by the apical shift of the dentogingival junction. As this occurs, the length of the clinical crown increases as the epithelial attachment migrates apically. Altered passive eruption occurs when the margin of gingiva is malpositioned incisally on the anatomic crown in adulthood and results in excessive gingiva. The purpose of this article is to evaluate esthetic results of crown lengthening procedure in altered passive eruption.s. MATERIALS AND METHODS: Three patients who complained "My front teeth look too short" were included. Bone sounding with periodontal probe revealed that alveolar bone crest was close to CEJ. Based on the diagnostic information, a diagnosis of altered passive eruption was made. They were performed apically positioned flap procedure with osseous resection. RESULTS: Six months later, all patients achieved favorable esthetic results and gingival margins were healthy and stable. CONCLUSION: When the diagnostic procedures reveal alveolar bone crest levels approximating the CEJ, apically positioned flap procedure with osseous resection is indicated.


Sujets)
Humains , Élongation coronaire , Couronnes , Attache épithéliale , Gencive , Dent , Col de la dent
3.
The Journal of the Korean Academy of Periodontology ; : 679-690, 2008.
Article Dans Coréen | WPRIM | ID: wpr-43835

Résumé

PURPOSE: The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA). MATERIAL AND METHODS: Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and 100 microgram/ml concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done. RESULTS: Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days. CONCLUSION: Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD.


Sujets)
Humains , Phosphatase alcaline , Numération cellulaire , Émail dentaire , Dentine , Acide édétique , Électronique , Électrons , Desmodonte , Tétracycline , Dent
4.
The Journal of the Korean Academy of Periodontology ; : 729-736, 2008.
Article Dans Coréen | WPRIM | ID: wpr-43829

Résumé

PURPOSE: Anterior ridge defect after tooth extraction results in unfavorable appearance. Ridge augmentation procedures should be preceded by careful surgical-prosthetic treatment planning, and various techniques can be used in anterior ridge augmentation. MATERIALS AND METHODS: Three patients showed deformed ridges after tooth extraction. Three different techniques ; onlay-interpositional connective tissue graft; bovine hydroxyapatite graft with free connective tissue graft; bovine hydroxyapatite graft with resorbable collagen membrane following free connective tissue graft; were used for anterior ridge augmentation. RESULT: Soft tissue graft can be used in small amount of ridge defect, hard tissue graft combined with soft tissue graft can be used in large amount of ridge defect. After ridge augmentation, about three months of healing period, augmented tissue was stabilized. The final restoration was initiated after this healing period, and the tissue form was maintained stable. CONCLUSION: Careful diagnosis and surgical-prosthetic treatment planning with joint consultation prior to surgery should be performed in order to attain an optimal esthetic results.


Sujets)
Humains , Collagène , Tissu conjonctif , Durapatite , Articulations , Membranes , Prothèses et implants , Extraction dentaire , Transplants
5.
The Journal of the Korean Academy of Periodontology ; : 621-628, 2008.
Article Dans Coréen | WPRIM | ID: wpr-157279

Résumé

PURPOSE: Osteopontin is one of the major non-collagenous protein of hard tissue. Use of peptide domain of biologically active protein has some advantages. The objective of this experimental study is evaluation of periodontal regenerative potency of synthetic peptide gel which containing collagen binding domain of osteopontin in the degree III periodontal defect of beagle dogs. MATERIAL AND METHODS: Experimental degree III furcation defect was made in the mandibular third and fourth premolar of beagles. Regenerative material was applied during flap operation. 8 weeks after regenerative surgery, all animals were sacrificed and histomorphometric measurement was performed to calculate the linear percentage of the new cementum formation and the volume percentage of new bone formation. RESULT: The linear percent of new cementum formation was 41.6% at control group and 67.1% at test group and there was statistically significant difference. The volume percent of new bone formation was 52.1% at control group and 58.9% at test group. CONCLUSION: As the results of present experiment, synthetic peptide gel containing collagen binding domain of osteopontin significantly increase new bone and cementum formation in the degree III furcation defect of canine mandible.


Sujets)
Animaux , Prémolaire , Collagène , Cément dentaire , Anomalies de furcation , Mandibule , Ostéogenèse , Ostéopontine , Structure tertiaire des protéines , Régénération
6.
The Journal of the Korean Academy of Periodontology ; : 859-869, 2007.
Article Dans Coréen | WPRIM | ID: wpr-24270

Résumé

Naked DNA and standard vectors have been previously used for gene delivery. Among these, PEI can efficiently condense DNA and has high intrinsic endosomal activities. The aim of this study is to investigate whether the cationic polycation PEI could increase the transfection efficiency of BMP expressing DNA using a vector-loaded collagen sponge model. BMP-2/pcDNA3.1 plasmid was constructed by subcloning human BMP-2 cDNA into the pcDNA3.1 plasmid vector. PEI/DNA complexes were prepared by mixing PEI and BMP-2/pcDNA3.1 and the constructed complexes were loaded into the collagen sponges. In vitro studies, BMSCs were transfected with the PEI/BMP-2/pcDNA3.1 complexes from collgen sponge. The level of secreted BMP-2 and alkaline phosphatase activities of transfected BMSCs were significantly higher in PEI/BMP-2/pcDNA3.1 group than in BMP-2/pcDNA3.1 group (p<0.05). Transfected BMSCs were cultured and mineralization was observed only in cells treated with PEI/BMP-2/pcDNA3.1 complexes. In vivo studies, PEI/BMP-2/pcDNA3.1/collagen, BMP-2/pcDNA3.1/collagen and blank collagen were grafted in skeletal muscle of nude mice. Ectopic bone formation was shown in PEI/BMP-2/pcDNA3.1/collagen grafted mouse 4 weeks postimplantation, while not in BMP-2/pcDNA3.1 grafted tissue. This study suggests that PEI-condensed DNA encoding for BMP-2 is capable of inducing bone formation in ectopic site and might increase the transfection rate of BMP-2/pcDNA3.1. As a non-viral vector, PEI offers the potential in gene therapy for bone engineering.


Sujets)
Animaux , Humains , Souris , Phosphatase alcaline , Collagène , ADN , ADN complémentaire , Thérapie génétique , Souris nude , Muscles squelettiques , Ostéogenèse , Plasmides , Porifera , Transfection , Transplants
7.
The Journal of the Korean Academy of Periodontology ; : 115-124, 2007.
Article Dans Coréen | WPRIM | ID: wpr-65887

Résumé

Silica is known as a promising osteoconductive material, and polycaprolactone is a bioactive and degradable material. The purpose of this study was to monitor the differentiation of MC3T3-E1 cells cultured on the layer-built silica/poly caprolactone non-woven fabric produced by electrospinning. Non-woven fabric (silica, polycaprolactone, PSP, SPS) was made by electrospinning and they were inserted in the 48 well cell culture plate. MC3T3-E1 cells were prepared by subculture. Cells were seeded to each well 1x10(5) concentration per well. Dulbecco's modified eagle medium with 10% FBS and 1% antibiotic-antimycotic solution was used. Confocal laser scanning microscope was taken 4 hours after incubation (95% air, 5% CO2, 37degrees C). Cell proliferation was monitored by spectrophotometer on 1, 7, 14 days, and the morphology of the growing cells was observed by field emission scanning electron microscope. To monitor the differentiation of osteoblasts on the materials, MC3T3-E1 cells were incubated in 48 well culture plate after seeding with the density of 1x10(5) concentration. Then ELISA kit & EIA kit were used on to assess osteocalcin and osteopontin expression respectively. The other conditions were the same as above. MC3T3-E1 cells were proliferated well on all of the materials. There were no statistical differences among them. The osteopontin expression of silica, PSP, SPS was significantly higher than other groups on day 3 (p<0.05), but after that time, there were no statistically signigicant differences. The osteocalcin expression was significantly higher in silica and PSP than other groups on day 14. These findings show that PSP was as good as silica on the effect of osteoblast differentiation. The PSP non-woven fabric may have the possibility as bone graft materials.


Sujets)
Techniques de culture cellulaire , Prolifération cellulaire , Aigles , Test ELISA , Ostéoblastes , Ostéocalcine , Ostéopontine , Silice , Transplants
8.
The Journal of the Korean Academy of Periodontology ; : 579-590, 2006.
Article Dans Anglais | WPRIM | ID: wpr-180211

Résumé

No abstract available.


Sujets)
Récepteurs de type Toll
9.
The Journal of the Korean Academy of Periodontology ; : 591-600, 2006.
Article Dans Coréen | WPRIM | ID: wpr-180210

Résumé

PURPOSE: This study assessed the impact of self-reported periodontal health on the oral health-related quality of life among elderly Koreans. METHODS: Four hundred twenty one elderly Koreans in Seoul and suburban areas were selected with a cluster (institution) sampling method, and were requested to take oral examinations and finish questionnaires on the Oral Health Impact Profile-14(OHIP-14), and self-reported periodontal health status, such as periodontal symptoms, self-rated periodontal health and periodontal treatment need. As the dependent variable, OHIP-14 showed a positive skewed distribution (skewness: 1.17), we transformed to square-root form to apply parametric analyses. Bivariate analysis by t-test and ANOVA, and multivariate analysis with the two-level regression model accounting clusters were implemented. RESULTS: Mean age of the subjects was 74.6 years and 66.5% were women. Fourteen items of OHIP-14 were summarized to one factor explaining 78.6% of total variance and produced the Chronbach alpha coefficient of 0.92. Results from the multivariate model, adjusting for age, sex, type of institutions, ability to pay, and number of teeth present, showed significantly lower OHIP-14 with reporting less than 3 periodontal symptoms (p<0.0001), rating their own periodontal health as above average level (p=0.0144), and thinking they don't need any periodontal treatments in the near future (p=0.0148), than their counterparts. The intraclass-corrrelation estimated by the final model was 0.028. CONCLUSION: This study demonstrates a significant association between self-reported periodontal health status and the oral health-related quality of life.


Sujets)
Sujet âgé , Femelle , Humains , Diagnostic buccal , Analyse multifactorielle , Santé buccodentaire , Qualité de vie , Enquêtes et questionnaires , Séoul , Pensée (activité mentale) , Dent
10.
The Journal of the Korean Academy of Periodontology ; : 555-565, 2006.
Article Dans Coréen | WPRIM | ID: wpr-27537

Résumé

The purpose of the present study is to evaluate the biological stability of the zirconia/alumina composite abutment by histologic and radiographic examination in clinical cases. 17 partially edentulous patients (5 men and 12 women, mean age 47) were treated with 37 implants. The implants were placed following the standard two-stage protocol. After a healing period of 3 to 6 months, zirconia/alumina composite abutments were connected. All radiographs were taken using paralleling technique with individually fabricated impression bite block, following insertion of the prosthesis and at the 3-, 6-, 12-month re-examinations. After processing the obtained images, the osseous level was calculated using the digital image in the mesial and distal aspect in each implant. An ANOVA and t-test were used to test for difference between the baseline and 3-, 6-, 12 months re-examinations, and for difference between maxilla and mandible. Differences at P 0.05). The mean bone level reduction in maxilla was 0.33(+/-0.25) at 3-months, 0.36(+/-0.33) at 6-months, 0.56(+/-0.26) at 12-months. And the mean bone level reduction in mandible was 0.35(+/-0.27) at 3-months, 0.49(+/-0.27) at 6-months, 0.68(+/-0.30) at 12-months. No statistical difference in bone level reduction between implants placed in the maxilla and mandible. Histologically, the height of the junctional epithelium was about 2.09 mm. And the width was about 0.51 mm. Scattered fibroblasts and inflammatory cells, and dense collagen network with few vascular structures characterized the portion of connective tissue. The inflammatory cell infiltration was observed just beneath the apical end of junctional epithelium and the area of direct in contact with zirconia/alumina abutment. These results suggest the zirconia/alumina composite abutment can be used in variable intraoral condition, in posterior segment as well as anterior segment without adverse effects.


Sujets)
Femelle , Mâle , Humains
12.
The Journal of the Korean Academy of Periodontology ; : 125-137, 2006.
Article Dans Anglais | WPRIM | ID: wpr-10076

Résumé

No abstract available.


Sujets)
Surfaçage radiculaire , Fumée , Fumer
13.
The Journal of the Korean Academy of Periodontology ; : 167-177, 2006.
Article Dans Anglais | WPRIM | ID: wpr-10072

Résumé

No abstract available.

14.
The Journal of the Korean Academy of Periodontology ; : 179-194, 2006.
Article Dans Anglais | WPRIM | ID: wpr-10071

Résumé

No abstract available.


Sujets)
Maladies parodontales
15.
The Journal of the Korean Academy of Periodontology ; : 39-49, 2006.
Article Dans Anglais | WPRIM | ID: wpr-170805

Résumé

No abstract available.


Sujets)
Animaux , Chiens , Durapatite , Transplants
17.
The Journal of the Korean Academy of Periodontology ; : 335-343, 2005.
Article Dans Coréen | WPRIM | ID: wpr-112941

Résumé

Inorganic bovine bone mineral has been widely researched as bone substitution materials in orthopedic and oral and maxillofacial application. OCS-B(NIBEC, Korea) is newly-developed inorganic bovine bone mineral. The aim of this study is to evaluate the safety and efficacy of bovine bone-derived bone graft material(OCS-B). Micro-structure of newly-developed inorganic bovine bone mineral(OCS-B) was analyzed by scanning electron microscope(SEM). Round cranial defects with eight mm diameter were made and filled with OCS-B in rabbits. OCS-B was inserted into femoral quadrant muscle in mouse. In scanning electron microscope, OCS-B was equal to natural hydroxyapatite. Rabbits were sacrificed at 2 weeks and 4 weeks after surgery and mice were sacrificed at 1 week and 2 weeks after surgery. Decalcified specimens were prepared and observed by microscope. In calvarial defects, osteoid and new bone were formed in the neighborhood of OCS-B at 2 weeks after surgery. And at 4 weeks after surgery osteoid and new bone bridge formed flourishingly. No inflammatory cells were seen on the surface of OCS-B at 1 week and 2 weeks in mouse experimental group. It is concluded that newly-developed inorganic bovine bone mineral(OCS-B) is a flourishing bone-forming material and biocompatible material.


Sujets)
Animaux , Souris , Lapins , Régénération osseuse , Durapatite , Orthopédie , Caractéristiques de l'habitat , Transplants
18.
The Journal of the Korean Academy of Periodontology ; : 427-436, 2005.
Article Dans Coréen | WPRIM | ID: wpr-204289

Résumé

OBJECTIVE: MMP-8 is a neutrophil enzyme and its level increases in some inflammatory diseases, including periodontal disease. We knew that the lipopolysaccharide of E.coli(E-LPS) induced MMP-8 release from human neutrophils. E-LPS is known to induce the production and release of inflammatory cytokines through CD14, Toll-like receptor(TLR). In the present study, we investigated whether MMP-8 release by E-LPS is induced via CD14-TLR pathway and the cellular mechanism of MMP-8 release in human neutrophils. MATERIAL AND METHODS: Human neutrophils were isolated from the peripheral blood of healthy donors and pre-incubated in medium containing antibodies against CD14, anti-TLR2 and anti-TLR4 or several inhibitors of microtubules and microfilaments and then incubated with E-LPS. The cells were treated TPCK and E-LPS simultaneously. The MMP-8amount in the culture medium was determined using ELISA. RESULTS: E-LPS increased MMP-8release from neutrophils and its induction was inhibited by anti- CD14 and anti-TLR4 but not by anti-TLR2 antibodies. The inhibitors of micro- tubule and microfilament polymerization significantly decreased E-LPS-induced MMP- 8release. TPCK inhibited E-LPS-induced MMP-8 release. CONCLUSION: These results suggest that MMP-8 release is induced by E-LPS via the CD14-TLR4 signal pathway in human neutrophils and may be depedent on microtubule and microfilament systems and NF-kappaB pathway.


Sujets)
Humains , Cytosquelette d'actine , Anticorps , Cytokines , Test ELISA , Lipopolysaccharides , Microtubules , Granulocytes neutrophiles , Facteur de transcription NF-kappa B , Maladies parodontales , Polymérisation , Polymères , Transduction du signal , Donneurs de tissus , Récepteurs de type Toll , N-(1-Benzyl-3-chloroacétonyl)-para-toluènesulfonamide
19.
The Journal of the Korean Academy of Periodontology ; : 511-524, 2005.
Article Dans Coréen | WPRIM | ID: wpr-204283

Résumé

The regeneration of lost periodontal tissue is a major goal of therapy. Periodontal ligament cell(PDL) is a specialized connective tissue that connects cementum and alveolar bone to maintain and support teeth in situ and preserve tissue homoeostasis. Bone morphogenetic proteins(BMPs) have shown much potential in the reconstruction of the periodontum by stimulate new bone and new cementum formation. Limitiations of BMP administration to periodontal lesions is high dose delivery, BMP transient biological activity, and low bioavailability of factors at the wound site. Gene delivery method can be alternative treatment strategy to deliver BMPs to periodontal tissue. The purpose of this study is to investigate efficiency of BMP-2 gene delivery with cell-based therapy using PDL cells. PDL cell were transduced with adenoviruses encoding either BMP-2 or Lac-Z gene. To evaluate osteogenic activity of expressed BMP-2 on PDL cells, we investigated secreted BMP-2, cellular activity, ALPase, produced mineralized nodules. To evaluate collagen scaffold as carrier for transduced cell delivery, we examined morphology and secreted BMP-2 of transducd PDL cells on it. BMP-2 transducd PDL cells produced higher levels of BMP-2, ALPase, mineralized nodules than non transduced cells. Cellular activity of transduced cells was showed similar activity to non transduced cells. Transduce cells attached on collagen scaffold secreted BMP-2 at 7day and was showed similar morphology to non transduced cells. These results demonstrated that transduced PDL cells produced biologically active BMP-2 and collagen scaffold could be carrier of transducd cells.


Sujets)
Humains , Adenoviridae , Biodisponibilité , Collagène , Tissu conjonctif , Cément dentaire , Desmodonte , Régénération , Dent , Plaies et blessures
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