Polymerase chain reaction-Based clonality analysis of cutaneous B-cell lymphoproliferative processes

Introduction: The differential diagnosis of B-cell lymphoproliferative processes remains a challenge for pathologists, dermatologists and oncologists, despite advances in histology, immunohistochemistry and molecular biology. Objective: Evaluate aid and limitations of clonality analysis in the diagnosis of primary cutaneous B-cell lymphomas and B-cell pseudolymphomas. Methods: This study included 29 cases of B-cell lymphoproliferative processes classified as primary cutaneous B-cell lymphomas (13), B-cell pseudolymphomas (6) and inconclusive cases (10) using histology and immunohistochemistry. The clonality analysis was performed by polymerase chain reaction analysis of immunoglobulin light chain and heavy chain rearrangements. Results: DNA quality was shown to be generally poor; eight samples were inadequate for polymerase chain reaction analysis. The results showed monoclonality in eight of the primary cutaneous B-cell lymphomas and polyclonality in four of the B-cell pseudolymphomas. In addition, monoclonality was shown in two of the inconclusive cases by histology and immunohistochemistry, demonstrating the utility of polymerase chain reaction as an ancillary diagnostic tool for primary cutaneous B-cell lymphomas. Discussion: The low quality DNA extracted from these cases demanded the use of an IgH protocol that yielded small fragments and IgK. Both methods used together improved detection. Conclusion: Use of the two protocols, immunoglobulin heavy chain FR3-trad and immunoglobulin light chain-Kappa Biomed protocols for clonality analysis improved diagnostic accuracy.

Fibrohistiocitoma maligno em criança, após tumor de Wilms: relato de caso / Malignant fibrous histiocytoma after WilmsÆ tumor: a case report

Malignant fibrous histiocytoma is a rare tumor in children, and its responsible for 2 to 6 percent of pediatric sarcomas. The authors report the case of a child who developed soft tissue malignant fibrous histiocytoma, as well as several fibrous histiocytic skin lesions, following treatment for WilmsÆ tumor patients has been improving very much over the last years because of successful treatment with chemo- and radiotherapy. A small proportion of these cases present a risk of developing a second neoplasm; genetic traits may enhance this risk. The authors discuss in this study those fibrous histiocytic lesions and the use of electron microscopy for definitive diagnosis. The authors conclude that follow-up of these patients treated for WilmsÆ tumor is of great importance for early detection of another neoplasm.

Feo hifomicose subcutanea por Exophiala jeanselmei: registro de tres casos em transplantados renais / Subcutaneous phaeohyhomycosis caused by Exophiala jeanselmei: report of 3 cases in kidney transplant patients

Sao registrados tres casos de feo-hifomicose subcutanea em transplantados renais provocados pela Exophiala jeanselmei (Langeron) McGinnis et Padhye 1977, fungo demacio capaz, tambem, de produzir raramente eumicetoma de graos pretos. Este fungo, segundo KWON-CHUNG&BENNETT, 1992 e antigenicamente muito heterogeneo, sendo identificados ate o presente momento tres sorotipos com subgrupos dentro de cada um deles....

Lúpus eritematoso sistêmico bolhoso: estudo de dois casos e revisäo da literatura / Bullous systemic Lupus erythematosus: study of 2 cases and literature review

Os autores apresentam dois casos de lúpus eritematoso sistêmico bolhoso confirmados clínica e laboratorialmente, juntamente com revisäo de literatura. Discutem a diagnose diferencial das dermatoses bolhosas juncionais associadas ao LES e caracterizam o LES bolhoso como entidade clínico-patológica

Tinha favosa: relato de ocorrência familiar em Itapecerica da Serra (Município da Grande Säo Paulo) / Tinea favosa: report of a familiar occurrence in Itapecerica da Serra (Municipality of the Greater Säo Paulo)

Os autores relatam 4 casos de tinha favosa por Trichophyton schoenleinii de ocorrência familiar, identificados na cidade de Itapecerica da Serra, município da Grande Säo Paulo. Todos os casos foram tratados com griscofulvina por via oral na dose de 10 mg/kg/dia por no mínimo 3 meses, tendo ocorrido cura clínica e laboratorial em 2 pacientes, e os dois restantes necessitaram de terapêutica mais prolongada