Sujets)
Animaux , Biopsie , Lignée cellulaire , Enfant , Modèles animaux de maladie humaine , Humains , Inde , Foie/cytologie , Cirrhose du foie/induit chimiquement , Souris , RatsRésumé
Methyl isocyanate (MIC), inhaled or administered subcutaneously (sc) at lethal concentration/dose caused essentially similar histopathological changes in all the viscera except for the lungs. The observed congestion of the viscera, foci of hepatocellular necrosis with widening of Disse's spaces in the liver and tubulo-rhexis with degeneration in the kidneys are attributable mostly to the initial shock. In addition, the lungs revealed more distinct route specific patterns of histopathological lesions. Inhaled MIC caused acute eosinophilic necrosis of the bronchial epithelium and frank alveolar edema, while MIC administered sc led to prominent vascular endothelial damage and severe interstitial pneumonitis with normal bronchial epithelium. The differential loci of damage in the lungs may be attributed to the immediate contact surface available for interaction with MIC.
Sujets)
Animaux , Isocyanates/administration et posologie , Rein/effets des médicaments et des substances chimiques , Foie/effets des médicaments et des substances chimiques , Poumon/effets des médicaments et des substances chimiques , Mâle , Rats , Rat Wistar , Rate/effets des médicaments et des substances chimiquesRésumé
M. mulatta monkeys were inoculated faeco-orally by enteric non-A, non-B virus to study the development of clinical, biochemical, histopathological and serological changes in the blood and liver. Pooled stool samples positive for putative non-A, non-B viral antigen by micro-ELISA and aggregated viral particles by immune electron microscopy, were administered in two M. mulatta monkeys. Biochemical, histopathological and serological changes were seen in the blood and liver and excretion of 27 nm virus like particles around 27 days of inoculation in the experimental monkey but not in the control animal.