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Southeast Asian J Trop Med Public Health ; 2003 ; 34 Suppl 2(): 121-2
Article Dans Anglais | IMSEAR | ID: sea-36342

Résumé

We report our experience with Gnathostoma protein preparation by the ultrafiltration method. Crude antigen was sonicated and ultrafiltrated using the Nanosep 100 K membrane. SDS-PAGE electrophoresis showed protein bands at 43, 41, 24, 22, 21, 19.5 kDa. Use of the ultrafiltration method can provide specific protein (24 kDa), similar to the non-ultrafiltration method, with the other 5 non-specific proteins. Using the non-ultrafiltration method, there were more (20) non-specific protein. The ultrafiltration method can be an alternative method for the preparation of protein, which can provide better results than non-ultrafiltration.


Sujets)
Animaux , Antigènes d'helminthe/isolement et purification , Mélanges complexes/composition chimique , Gnathostoma/immunologie , Protéines d'helminthes/isolement et purification , Membrane artificielle , Nanotechnologie , Ultrafiltration
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