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In this study, the rapid resolution liquid chromatography-quadrupole time-of-flight mass spectrometry (RRLC-QTOF/MS) was used to profile the metabolites of urine samples from chronic heart failure (CHF) patients and healthy controls to find the differential metabolites which could provide the scientific evidence to explain the pathogenesis of the disease and supply a better therapy plan.Urine samples from 15 CHF patients (age (62.27±3.14) years) and 15 healthy controls (age (65.41±4.63) years) were analyzed by RRLC-QTOF/MS.After processing the data, the multivariate statistical analysis (principal component analysis, PCA) was performed to find the potential biomarkers.Result showed that urine samples of CHF patients were successfully distinguished from those of healthy controls.Two significantly differentially expressed metabolites, uridine and alanyltryptophan, were found and identified as potential biomarkers.The result showed that the LC-MS based metabolomics approach had good performance to identify potential biomarkers, and the disorder of uracil metabolism and Tryptophan metabolism may play an important role in the mechanism of CHF.
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A rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometric ( RRLC-QTOF/MS) method was used to profile the metabolites of urine samples from atherosclerosis ( AS) patients and healthy controls and find the differential metabolites which could provide the scientific evidence to explain the pathogenesis and early disease diagnose. In the study, 15 AS patients ( age46. 84±2. 41 years) and 15 healthy controls ( age45 . 72±1 . 93 years ) was screened out by VaSera VS-1000 . The urine samples were analyzed by RRLC-QTOF/MS and the resulting data matrices were analyzed by multivariate statistical analysis ( Principal Component Analysis, PCA ) to find the potential biomarkers. The results showed that the urine samples of AS patients were successfully distinguished from those of healthy controls. Besides, a total of two significantly changed metabolites, uric acid and Guanidineacetic acid, had been found and identified as potential biomarkers, which suggested that the disorder of purine metabolism and amino acid metabolism played an important role in the mechanism of AS.
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Objective To investigate the clinical effects of Losartan on hemorrheology and left heart function in essential hypertensive patients.Methods 43 essential hypertensive patients were treated with Losartan,50 ms/d～100 ms/d for twelve to seventeen months.20 essential hypertensive patients were treated with Fu Fang Jiang Ya Pian as control group.Then the changes of hemorrheology indexes and left heart function were tested in both groups.Results After the treatment with Losartan the blood pressure reduced effectively(P＜0.001),the whole blood low shear rate hyperviacoSity(ηb),plasma viscosity,AI,whole blood low shear rate reduced viscosity,erythrocyte electrophoresis,Fib reduced obviously(P＜0.01),but the control group had not statistical significance(P＞0.05)before and after treatment.The whole blood high shear rate hyperviscesity,whole blood high shear rate reduced viscosity,Hct,RRI reduced too(P＞0.05),but there was no statistical significance.LVM(P＜0.01)and LVMI(P＜0.05)were obviously reduced,E/A was increased(P＜0.01),IRT(P＜0.05)was shorten (P＜0.05),while the cardiac diastole function improved effectively.The significant differences were not observed in the control group pre and pest treatment(P＞0.05).Condusion Losartan can effectively reduce blood pressure.At the same time,it improves RBC deformation capability,reduce erythrecyte aggregation,Fib,Hct of essential hypertensive patients,and reduce blood viscosity.Losartan can also reduce myocardial hypertrophy,heart weight,and improve heart function.
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Objective To study the extracellular signal-regulated kinase 1/2(ERK1/2) expression of renal cells in epilepsy rats induced by kainic acid,and elucidate the mechanism of renal injury caused by epilepsy.Methods 70 male Wistar rats were randomly divided into two groups:epilepsy group and control group,35 rats in each group.Rat epilepsy model was prepared by injection of kainic acid into amygdala under three-dimensional positioning devices.At different time points(0,2,6,12 and 24 h) after epilepsy spasm,the renal tissue specimens were immediately prepared for immunohistochemistry.The ERK1/2 expression of rat renal cells was calculated and compared with control group.Results The ERK1/2 expression(gray value) of renal tubular epithelial cells in epilepsy rats began to increase gradually after epilepsy spasm.It reached peak at 6 h after epilepsy spasm.The ERK1/2 expression of renal tubular epithelial cells in epilepsy rats was significantly higher than at 0 h(P0.05).There were no major pathological changes in kidney of rats with epilepsy stained with HE.Conclusion ERK1/2 activation may have important role in the renal injury caused by epilepsy.Inhibition of the activation of ERK1/2 may lead to the renal protection.
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Objective To disccuss the antioxidative effects of sodium ozagrel(OZG) in myocardial ischemia in rats and illuminate its effect of decreasing oxidative damage.Methods Forty healthy Wistar rats were randomly devided into five groups:control,model,pretreatment,treatment and therapeutic alliance groups.OZG was used for 2 weeks in pretreatment group,then isoprenaline hydrochloride (ISO) was applied to make myocardial ischemia model.5 min after the establishment of model,OZG and OZG combined with glyceryl trinitrate(GT) were used in treatment group and therapeutic alliance group,respectively,ECG was recorded at the same time.1 h later,the activities of serum myocardial creatases(AST,LDH,CK),malonaldehyde(MDA) level and superoxide dismutase(SOD) activity were detected.Results ECG:When the model was successfully set up,ST segment in every group all raised≥0.1 mV,the minimum value of raise was in pretreatment group.When the model was succeeded 5 min later,ST segment in model group slightly decreased but still ≥0.1 mV and it was significant in pretreatment group.In treatment and therapeutic alliance groups,compared with model succeeding'ST segment,ST segment decreased significantly at 5 min later(P
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Objective To study the effect of candesartan on the ERK1/2 protein expression in myocardium of epilepsy rats induced by kainic acid(KA) and its mechanism.Methods 105 male Wistar rats were divided into: control group(A);epilepsy groups(B1-5);candesartan groups(C1-5);1-5 meaned 0,0.5,2,4,6 h after epilepsy,respectively.Epilepsy rat models were made by injecting KA into amygdale under stereotactic instrument.The ERK1/2 protein expression in various groups were tested with immunohistological method.Results Compared with control group,the protein expressions of ERK1/2 increased significantly in the 0.5 h groups of B and C(P0.05).The differences in the level of ERK1/2 protein expression between B and C groups were significant(P
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Objective To study the relationship between plasma lipids and gallbladder stones(GD).Methods The plasma lipids levels were determined and GD was diagnosed by ultrasonic instrument in 5 982 subjects,then the occurrence of GD was compared between hyperlipemia and no-hyperlipemia.Results The detectable rate of GD of the hyperlipemia was 9.86% and that of the no-hyperlipemia was 7.00%,the difference was significant(P0.05,OR=1.223,95%CI:1.127-2.748).Conclusion The prevalence rate of GD in hyperlipemia patients is higher than that in normal plasma lipids subjects.The sick risk of GD in patients with hypercholesterolemia is much higher than that in persons with normal total cholesterol levels.Sex has no influence on the formation of GD in hyperlipemia patients.