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1.
Braz. j. microbiol ; 46(2): 443-453, Apr-Jun/2015. tab, graf
Article Dans Anglais | LILACS | ID: lil-749707

Résumé

The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented by A. pallidus (4) and Aeribacillus sp. (2). In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.


Sujets)
Bacillaceae/classification , Bacillaceae/isolement et purification , Bâtonnets à Gram positif/classification , Bâtonnets à Gram positif/isolement et purification , Sources thermales/microbiologie , Microbiologie du sol , Microbiologie de l'eau , Biodiversité , Bacillaceae/génétique , Bacillaceae/effets des radiations , Analyse de regroupements , ADN bactérien/composition chimique , ADN bactérien/génétique , ADN ribosomique/composition chimique , ADN ribosomique/génétique , Bâtonnets à Gram positif/génétique , Bâtonnets à Gram positif/effets des radiations , Données de séquences moléculaires , Maroc , Phylogenèse , /génétique , Analyse de séquence d'ADN , Spores bactériens/cytologie
2.
Mem. Inst. Oswaldo Cruz ; 106(4): 394-399, June 2011. ilus, tab
Article Dans Anglais | LILACS | ID: lil-592180

Résumé

An online scheme to assign Stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (MLSA), which is based on the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA), the recombination repair protein (recA), the RNA polymerase alpha subunit (rpoA) and the excision repair beta subunit (uvrB). This MLSA-based scheme was validated using eight of the 10 Stenotrophomonas species that have been previously described. The environmental and nosocomial Stenotrophomonas strains were characterised using MLSA, 16S rRNA sequencing and DNA-DNA hybridisation (DDH) analyses. Strains of the same species were found to have greater than 95 percent concatenated sequence similarity and specific strains formed cohesive readily recognisable phylogenetic groups. Therefore, MLSA appeared to be an effective alternative methodology to amplified fragment length polymorphism fingerprint and DDH techniques. Strains of Stenotrophomonas can be readily assigned through the open database resource that was developed in the current study (www.steno.lncc.br/).


Sujets)
Humains , ADN bactérien , Typage par séquençage multilocus/méthodes , Stenotrophomonas , Techniques de typage bactérien , DNA-directed RNA polymerases , Phylogenèse
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