Study of antioxidant effect of selenium on female rats treated with contraceptives or estrogen replacement therapy

Selenium is an essential trace mineral in the human body. it is an important part of antioxidant enzymes [Glutathione peroxidase] that protects cells against the effect of free radicals. Contraceptives are capable of inhibiting ovulation and successfully control fertility. Estrogen replacement therapy [ERT] is a wide spread treatment in postmenopausal women to alleviate climatic complaints. It has also been applied in the prevention of osteoporosis. The present study aims to follow-up antioxidant enzymes status of normal adult and ovariectomized female albino rats treated with contraceptive. Selenium was orally administered in therapeutic dose of 50 micro g/kg.b.wt/day, oral contraceptives [estrogen 0.54 and progesterone 2.7 micro g/kg.b.wt/day] and intramuscular injection of ERT 90 micro g/kg.b.wt/day for 30 days. Repeated medication with selenium caused very highly significant increase in super-oxide dismutase [SOD], reduced glutathione [GSH], glutathione reductase [GSH-R], glutathione S-transferase [GST] and glutathione peroxidase [GSH-PX] and decline in lipid peroxidation [LPO]. Contraceptives and ERT caused significant reduction in SOD, GSH, GSH-R, GST, GSH-PX and significant increase in LPO. When selenium was administered with contraceptive or with ERT, a slight increase was noticed in the different antioxidant parameters studied except for LPO which recorded a significant decrease.

Effect of selenium on lipid profile of rats treated with contraceptives or certain hormone replacement therapy

Selenium play a vital part in many metabolic functions while new researches increasingly suggest it is relevance to disease prevention. Contraceptive are capable of inhibiting ovulation and successfully controlling fertility. Estrogen is a wide spread treatment in post menopousal women to alleviate climatic complaints. The present study planned to achieve three goals. First one: aims to investigate some biochemical study on lipid content and side effect that occur from repeated administration of drugs [selenium, contraceptive and estrogen replacement therapy] in experimental animals. Follow up the effect on repeated administration [accumulation doses] of contraceptive on rats. Second the effect of selenium on antioxidant to amelioration any hardard effects if present. Third of them of lipid profile. Selenium was orally administered in therapeutic dose [50 micro g/kg/day], contraceptive [estrogen 540 micro g/kg/day, progesterone 2.7 micro g/kg/day] and estrogen replacement therapy [90 micro g/kg/day] to adult female albino rats ovarectomized female rats for 30 days. Repeated medication with Selenium caused significant reduction in total lipid [T.L], Triglycerid, [T.G.], total cholesterol [T.Ch.], HDL, cholesterol, LDL cholesterol and serum estradiol. Contraceptives and estrogen replacement therapy revealed very highly significant increase in T.L, T.G. T.Ch, HDL cholesterol, LDL cholesterol and estradiol When selenium was administered with contraceptives or estrogen replacement therapy, noticeable amelioration was recorded in lipid profile a slight increase was noticed in the different studied lipid parameters.