Résumé
BACKGROUND: Whartons jelly-derived mesenchymal stem cells are a valuable alternative source that possess multipotent properties, easy to obtain and available in large scale compared to BMMSCs. We investigated the possibility of cardiac function improvement post isoproterenol induced cardiac injury in a rat model following human WJMSCs transplantation. MATERIALS AND METHODS: MSCs were extracted and cultured from cord WJ, characterized by morphology, Immunophenotyping and differentiation to osteoblast and adipocytes. WJMSCs were labeled with PKH2 linker dye. Wistar rats were divided into control group, ISO group (injected with 2 doses of isoproterenol) to induce myocardial injury and ISO group transplanted with labelled WJMSCs. ECG, electrocardiographic patterns, cardiac marker enzymes, tracing of labeled MSCs and immunohistochemical analysis of myocardial cryosections were studied. RESULTS AND CONCLUSIONS: WJ derived MSCs were expanded for more than 14 passages while maintaining their un-differentiated state, were positive for MSC markers and were able to differentiate into adipocyte and osteoblast. We demonstrated that intravenously administered WJMSCs were capable of homing predominently in the ischemic myocardium. Cardiac markers were positively altered in stem cell treated group compared to ISO group. ECG and ECHO changes were improved with higher survival rate. WJMSCs could differentiate into cardiac-like cells (positive for cardiac specific proteins) in vivo. WJMSCs infusion promoted cardiac protection and reduced mortality, emphasizing a promising therapeutic role for myocardial insufficiency.
Sujets)
Humains , Adipocytes , Électrocardiographie , Immunophénotypage , Isoprénaline , Transplantation de cellules souches mésenchymateuses , Cellules souches mésenchymateuses , Modèles animaux , Mortalité , Myocarde , Ostéoblastes , Rat Wistar , Rodentia , Cellules souches , Taux de survie , Transplantation , Gelée de WhartonRésumé
To determine the possible role of MP in exacerbations of asthmatic attacks in children,we studied 43 cases during acute attacks by throat swab culture and sera testing for IgMantibodies to MPby ELISA.MPwas isolated in 3 cases and IgM to MP was high in 8 episodes. The study shows that MP although not commonly isolated may be important in exacerbating acute asthmatic attacks in some children