Résumé
Objective To establish the HPLC fingerprint of Xuanbo Shuangsheng Granule for its quality control.Methods The components were separated on an Agilent TC-C18 column (150 mm ×4.6 mm,5 μm) at 25 ℃,with a gradient elution in 0-60 min at the flow rate of 1 mL/min using 0.2％ acetic acid aqueous solution and methanol as the mobile phase.The detection wavelength was 278 nm.By detecting 11 batches of Xuanbo Shuangsheng Granule,the HPLC fingerprint was established using Similarity Evaluation System for Chromatographic Fingerprint of TCMs (Version 2004A)and the common peaks were analyzed and identified in raw herbal material.Results The HPLC fingerprint of Xuanbo Shuangsheng Granule was obtained with similarity all over 0.9.Totally 27 common peaks were confirmed,and each common peak could be found in raw herbal medicines.Based on the reference substances,5 common peaks were identified,including phellodendrine (peak 11),liquiritin (peak 22),angoroside C (peak 25),cinnamic acid (peak 26) and harpagoside (peak 27).Conclusions This method is simple,accurate,repeatable and reliable,which could be applied in the quality control of Xuanbo Shuangsheng Granule.
Résumé
OBJECTIVE:To develop an RP-HPLC method to determine the camphor in Bohenao zhangnao nose drops. METHODS:The HPLC separation was carried out on a Supelcosil C18(150 mm?4.6 mm,5 ?m) column.The mobile phase consisted of methanol-water(70∶30) at a flow rate of 1 mL?min-1.The column temperature was 25 ℃ and the detective wa-velength was set at 289 nm. The content was computed using external standard method. RESULTS:The linear range of cam-phor was 0.1～1 mg?mL-1(r=0.999 8) with recovery rate ranged from 100% to 103%(RSD