Résumé
In this paper we carried out a study about prevalence of the clinically significant coagulase negative staphylococcal (CNS) isolates found in an university hospital. Two hundred four CNS isolates from 191 patients obtained between the period of 1998 to 2002, were studied. About 27 percent (52/191) of the infection cases studied were confirmed as CNS-associated diseases. Blood stream infection (BSI) was the most frequent CNS associated-disease (25 percent; 13/52). The great majority of the BSI was verified in the Neonatal Intensive Care Unit (NICU). The analysis of the 52 patients medical history showed that 85 percent of the BSI was acquired in hospital. Most of the CNS nosocomial infections were associated with the use of indwelling medical devices. The incidence of methicillin-resistance among significant CNS isolates was 38 percent. In this study, a high percentage of exogenous contaminant was verified (60 percent), indicating that contamination of clinical specimens during sample collection is critical.
Sujets)
Humains , Animaux , Bactéries à Gram négatif/isolement et purification , Études transversales , Coagulase/analyse , Coagulase/isolement et purification , Infections bactériennes à Gram négatif , Infection de laboratoire , Infections à staphylocoques , Staphylococcus/isolement et purification , Staphylococcus/pathogénicité , Techniques et procédures diagnostiques , Méthodes , Méthodes , VirulenceRésumé
O meio LAL-1, descrito recentemente (14), foi modificado pela adiçäo de 15 ug/ml de ácido nalidíxico (meio LAL-2). OLAL-2 permitiu a detecçäo de todos os GBS isolados de mulheres altamente colonizadas pelo microrganismo. O meio modificado foi bastante eficiente para o enriquecimento de GBS visto que a detecçäo do microrganismo aumentou em aproximadamente 57