Résumé
The results obtained and the laboratory methods used for the isolation of viruses and bacteria from Malaysian children with acute respiratory illnesses seen in a private clinic are described. Of the 65 children studied virus isolations were obtained from 26 children, bacteria from 10 and both virus and bacteria from another 5. The agents isolated were influenza viruses, parainfluenza viruses, adenoviruses, Bordetella pertussis, Streptococcus pneumoniae, Haemophilus influenzae and Staphylococcus pyogenes.
Sujets)
Maladie aigüe , Adenoviridae/isolement et purification , Facteurs âges , Bactéries/isolement et purification , Bordetella pertussis/isolement et purification , Enfant , Enfant d'âge préscolaire , Humains , Orthomyxoviridae/isolement et purification , Infections de l'appareil respiratoire/microbiologie , Respirovirus/isolement et purification , Staphylococcus/isolement et purification , Streptococcus pneumoniae/isolement et purification , Virus/isolement et purificationRésumé
A rapid and simplified system for the differentiation of nonfermentative Gram-negative bacilli, encountered frequently in clinical specimens, is presented for use in the clinical laboratory. Nonfermentative bacteria can be grouped initially by the motility, oxidase and OF glucose reactions. This grouping simplifies the choice of additional tests for further identification. The additional tests included Gram stain, acid production from 10% lactose agar, nitrate reduction, arginine dihydrolase activity, fluorescein production, deoxyribonuclease activity, hydrolysis of aesculin, growth at 42 degrees C, gelatinase activity and susceptibility to antibiotics.
Sujets)
Acinetobacter/métabolisme , Alcaligenes/métabolisme , Bactéries/métabolisme , Techniques bactériologiques , Milieux de culture , Flavobacterium/métabolisme , Glucose/métabolisme , Humains , Moraxella/métabolisme , Pseudomonas/métabolismeRésumé
Three strains of Pseudomonas putrefaciens were isolated from routine clinical specimens at the University Hospital, Kuala Lumpur, Malaysia. Their cultural and biochemical characteristic, and antibiotic susceptibilities are presented. Characteristics of diagnostic value were stressed. Two isolates appeared to have played a pathogenic role in chronic otitis media.
Sujets)
Adulte , Femelle , Humains , Mâle , Adulte d'âge moyen , Otite moyenne/microbiologie , Pseudomonas/effets des médicaments et des substances chimiques , Infections à Pseudomonas/microbiologie , Infections de l'appareil respiratoire/microbiologieRésumé
Simultaneous MIC determinations were performed by the conventional tube dilution method and a microdilution technique. Forty-five clinical isolates and 11 antibiotics were used. Results of the two tests revealed 89.2% aggrement of +/- one dilution. The microdilution method was found to be reliable, reproducible and economical in time, labour and materials. The use of pH indicator in the microtechnique is recommended for certain organisms.