Résumé
OBJECTIVE: To study the effects of 3, 4-di-O-methyl-1, 2, 5, 6-D-mannitol tetranitrate(DMMTN) on acute myocardial ischemia induced by ligating coronary artery in rats. METHODS: Acute myocardial ischemia model was developed by ligating the anterior descending branch of left coronary artery in rats. The rats were randomly divided into 6 groups: sham group, model group, Lunanxinkang group, low-dose (15 mg · kg-1) DMMTN group, middle-dose (30 mg · kg-1) DMMTN group and high-dose (60 mg · kg-1) DMMTN group. The changes of ST segment and T segment of electrocardiogram at different moment were recorded after myocardial ischemia injury. At 3 h after ligation, the myocardial infarct size was measured by nitroblue tetrazolium (NBT) staining method. And the activities of creatine phosphokinase (CK), lactate dehydrogenase (LDH) and superoxide dismutase (SOD), and the content of malondialdehyde (MDA) were determined to evaluate the protective effects of DMMTN. RESULTS: DMMTN could reduce the size of myocardial infarct significantly, inhibit the rising of ST segment and T segment of electrocardiogram, decrease the level of CK, LDH and MDA, and increase the activity of SOD. CONCLUSION: DMMTN showed obvious protective effects on acute myocardial ischemia induced by ligating the coronary artery in rats. Copyright 2012 by the Chinese Pharmaceutical Association.