RÉSUMÉ
Objective To construct the recombinant adenovirus vector carrying mouse β-nerve growth factor (β-NGF) labeled with green fluorescent protein (GFP). Methods Adult healthy male Kunming mice, weighting (60-70) g, were chosen in our study. The full-length β-NGF cDNA was amplified from the total mRNA of mouse submandibular gland by RT-PCR, and then, this fragment was cloned into the adenovirus shuttle plasmid pAdtrack-CMV (marked with GFP); and then, the pAdeasy-1/pAdtrack-CMV-β-NGF was constructed with defective adenovirus genome pAdeasy-1 by homologous recombination in bacteria; at last, restriction analysis was performed on this adenovirus vector pAdeasy-l/pAdtrack-CMV-GFP-β-NGF.Results Amplified full-length mouse β-NGF fragment was obtained by RT-PCR; the pAdTrack-CMV-β-NGF contained the target gene; double digestion indicated that the recombinant plasmid pAdTrack-CMV-β-NGF was successfully constructed;restriction enzyme digestion analysis indicated that pAdeasy-l/pAdtrack-CMV-βNGF adenovirus vector by homologous recombination was constructed. Conclusion The recombinant adenovirus vector pAdeasy- 1/pAdtrack-CMV-GFP-β-NGF is successfully constructed.
RÉSUMÉ
Objective To investigate the effect of endovascular interventional therapy on intracranial aneurysm.Methods We retrospectively analyzed the clinical information and treatment efficacy of 48 patients (53 aneurysms) experienced interventional embolism therapy; these patients were admitted to and received treatment in our hospital from January 2001 to December 2009.Results Among the 53 aneurysms of 48 patients,40 aneurysms were obliterated completely,6 aneurysms 95％ obliterated,5 aneurysms 90％ obliterated and 2 aneurysms failed; 2 aneurysms ruptured and no death was noted during the operation.Six to 12 months after the operation,follow-up of the 46 patients indicated that 2 were recurred under CMA or DSA; 2 was severely disabled; 5 had mild neurological deficits; and the other enjoyed good results.Conclusion Endovascular embolization ofaneurysms is a minimally invasive method with low risk; individualized embolism therapy can improve the prognosis.