Résumé
ascorbic acid plays a critical role in wound repair and healing/regeneration process as it stimulates collagen synthesis. Its effect on jejunal and colonic anastomosis is already established. We assessed its role on colonic anastomosis healing in presence of peritonitis and without peritonitis. Assessment was done by recording bursting pressure of anastomosis and histological features of healing.METHODSThis experimental study was conducted in 48 Albino Rats (mean weight of 180 g). All rats were subjected to exploratory laparotomy with colonic resection anastomosis and were divided into six groups. Group A and B control, Group C and D were supplemented with Vitamin C in low dose i.e. 100 mg/Kg body, Group E and F high dose of Vitamin C i.e. 200 mg/Kg body wt. Vitamin C was given for seven days intra muscularly. Peritonitis was created by cecal ligation and puncture (CLP) in half the number of rats (n=24), in each group. Every rat was re-explored on 7th post-operative day and anastomotic segments were harvested for bursting pressure measurement and then sent for histopathology examination.RESULTSBursting Pressure of group D (100 mg) was significantly raised (p<0.05) as compared to group B in the presence of peritonitis. Group F (200 gm vit C) showed significantly higher bursting pressure than group D (p<0.05). The findings of analysis of variance (ANOVA) showed that the mean BP of all the groups differs significantly from each other (p<0.05). High dose vitamin C supplementation gave more strength to anastomosis than low dose even in presence of peritonitis. The average histopathological examination score was 5.24 (Group E) (p<0.05). i.e., best healing among all groups. It had better effect even in presence of peritonitis - 4.62 in Group F. Healing score in this group was greater than group A, B, C, D and less than in group E.CONCLUSIONSPresence of fecal peritonitis hampers the process of healing of colonic anastomosis by impairing reparative collagen synthesis, its deposition and anastomotic strength reflected by decreasedbursting pressure and decrease HPE score. Supplementation of systemic ascorbic acid increases intestinal anastomotic healing by increasing collagen accumulation reflected by increasedbursting pressure and increase HPE score even in presence of peritonitis. High dose ascorbic acid supplementation had better effect on intestinal anastomotic healing. Importance of ascorbic acid in colonic anastomosis healing is yet to be evaluated in human beings.
Résumé
The current study was undertaken to compare the effects of pulmonary oedema producing toxin (PO-Tx) isolated from Mesobuthus tamulus venom on cardio-respiratory reflexes with exogenously administered bradykinin (BK) and to delineate the type of BK receptors mediating these responses. Jugular venous injection of phenyldiguanide (PDG) in anaesthetized rats produced reflex bradycardia, hypotension and apnoea. The PDG-induced reflex was augmented (two folds) by PO-Tx. The pulmonary water content in POTx treated group was also increased. The PO-Tx-induced reflex changes as well as pulmonary oedema were blocked by Hoe-140 implicating the involvement of B2 kinin receptors. Exogenous BK also produced augmentation (two folds) of the PDG-induced reflexes and increased the pulmonary water content. The BKinduced augmentation was blocked by pre-treatment with des-Arg10 Hoe 140 (a B1 receptor antagonist) and Hoe 140 (B2 receptor antagonist). However, these antagonists did not prevent the development of BK-induced pulmonary oedema. Present results indicate that PO-Tx augmented the PDG-induced reflex responses similar to BK and the PO-Tx induced augmentation of reflexes is mediated through B2 receptors.
Résumé
The study was undertaken to evaluate the efficacy of multivitamin and micronutrient supplementation in azoospermic patients with maturation arrest. A total of 35 azoospermic patients showing maturation arrest on testicular biopsy were recruited in this study. The patients were divided into two groups. Untreated group (n=11) without any treatment and treated group (n=24) who received multivitamins, micronutrients and co-enzyme Q10. The sperm concentration, motility and morphology were evaluated at monthly interval. The results showed reduction in liquefaction time and relative viscosity of the semen in the treated group. Further, in treated group there was appearance of spermatozoa (4.0 million/ml) exhibiting progressive motility (7%) and normal morphology (6%), even in the first follow up visit. The sperm count, motility and normal morphology increased significantly on subsequent visits. Within 3 months (3 visits) 2 pregnancies were reported. These observations indicate that multivitamin and micronutrient supplementation improve the qualitative and quantitative parameters of seminogram in patients with azoospermia of maturation arrest.