Effect of adrenaline administration on the histological structure, lipid and ascorbic acid contents of mouse adrenal cortex

The adrenal glands were taken from 9 male mice after a single intraperitoneal injection of 0.03/100 g body weigh of adrenaline hydrochloride solution at different periods of time 0.5 hour, one hour and 2 hours [3 at a time]. The histological structure, lipid ascorbic acid contents have been studied. The results obtained in the present study showed that, the most obvious changes were seen as follows: Dilatation of cortical sinusoids, loss of subglomerulosal zone, and transformation of the fasciculate cells from the clear to dark type. Depletion of sudanophilic materials from zona reticularis and zona fasciculata started at 0.5 hour after injection with maximal depletion at 2 hours after injection. A single adrenaline injection caused a rapid fall in ascorbic acid content at 0.5 hour after injection. One hour after injection there was complete ascorbic acid depletion from zona glomerulosa and outer zona fasciculata

Effect of cortisone administration on the cholesterol content and phosphomonoesterases activities of mouse cortex

After a single IM injection of cortisone, the adrenal glands of 9 male mice were taken at different intervals of time 3 hours, 2 days and 3 days [3 at a time]. The cholesterol content and phosphomonoesterases activities have been studied. The results obtained in the present study showed that, 3 hours after cortisone injection, depletion of cholesterol content was observed in both zona fasciculata and zona reticularis. Subsequent accumulation of cholesterol in the zone fasciculata then occurred. Cortisone injection caused a decrease in phosphomonoesterases activities 3 hours after the injection, but these activities increased after 2 days from the injection

Effect of anoxia and heat on the ascorbic acid content and phosphomonoesterases actives of mouse adrenal cortex

9 male mice were included in the present study. They were kept in individual small cages in an oven of constant temperature at 45C with low oxygen content, then the adrenal glands were taken at periods of time [5 minutes, 10 minutes and 15 minutes]. The ascorbic acid content and the activities of phosphomonoesterases have been studied. The results obtained in the present study showed that, ascorbic acid was sensitive to heat and anoxia, since 5 minutes after exposure, a decrease of positive granules was observed from the superficial zones of the cortex to be concentrated in the inner zones, which showed a transport of these granules from the cells through the sinusoids. Alkaline phosphatase activity did not show any change all over this study. Initial increase in acid phosphatase after 5 minutes of exposure to heat and anoxia followed by gradual decrease in enzyme activity