Résumé
We report here the case of a 75-year-old lady who presented to us with a 4-month history of abdominal symptoms. The computed tomography scan revealed a cystic lesion in the tail of the pancreas. Distal pancreatectomy was done and biopsy showed a benign mucinous neoplasm. Because this is potentially malignant it is vital to diagnose it before it becomes malignant; identification of this entity remains a diagnostic and therapeutic challenge.
Sujets)
Sujet âgé , Cystadénome mucineux/anatomopathologie , Femelle , Humains , Tumeurs du pancréas/anatomopathologieRésumé
Mucinous carcinoma of the male breast is a very rare tumour. So far only about 8 cases have been reported in the literature most of which were diagnosed after surgical resection. Fine-needles aspiration biopsy even though a well established procedure for the evaluation of breast masses in female there are only occasional reports of aspirates form male breast masses. We herein-present one case of mucinous carcinoma of breast in a 65 year old male diagnosed by fine needle and confirmed by histopathology.
Sujets)
Adénocarcinome mucineux/diagnostic , Sujet âgé , Ponction-biopsie à l'aiguille , Tumeur du sein de l'homme/diagnostic , Humains , MâleRésumé
This work was carried out to study the modulation of arginase expression in experimental diabetes. Here, we have demonstrated that liver arginase activity and mRNA levels increased significantly in diabetic condition. Insulin treatment reverses the increased activity and mRNA levels nearly to the control values. The reversal effects of vanadate are found to be similar to that of insulin and this observation further reiterates the insulin-like effects of vanadate. ELISA and slot blot assay observations are consistent with biochemical measurements of enzyme activity. These results show an increase in arginase activity and mRNA levels in diabetes and decrease in treated animals may be due to the transcriptional regulation of arginase gene.
Sujets)
Animaux , Arginase/métabolisme , Diabète expérimental/traitement médicamenteux , Femelle , Insuline/pharmacologie , Foie/effets des médicaments et des substances chimiques , ARN messager/métabolisme , Rats , Rat Wistar , Vanadates/pharmacologieRésumé
Ribulose-1,5-bisphosphate carboxylase has been isolated from a synthetic cereal triticale and purified using a newly developed rapid procedure involving precipitation with ammonium sulphate (35-55% saturation), DEAE-cellulose (DE-52) chromatography and filtration through Sepharose CL-68. Molecular weights of the enzyme subunits are 15.5 and 52 kDa which corresponds to 540 kDa for the hexadecameric holoenzyme. Isoelectric focussing showed that the enzyme has a pI of 4.2. Various kinetic constants determined under aerobic conditions are: Km (CO2), 118 microM; Km (RuBP), 220 microM (at 20 mM NaHCO3) and Vmax, 690 nmole CO2 fixed/mg enzyme/min.
Sujets)
Cations , Grains comestibles/enzymologie , Chromatographie sur gel , Électrophorèse sur gel de polyacrylamide , Activation enzymatique , Cinétique , Masse moléculaire , Ribulose bisphosphate carboxylase/composition chimique , Sels/pharmacologieRésumé
Nuclear DNA isolated from hypocotyls (H), proliferating callus (PC) and differentiating callus (DC) of Brassica juncea contains a satellite DNA which can be resolved in actinomycin-D/CsCl gradients. The satellite DNA undergoes changes, when an in vitro culture is raised from hypocotyl tissue and forms a higher percentage of the genome in PC and DC than in mature differentiated tissue (hypocotyl). All the three satellite DNAs are GC-rich compared to main band DNAs. Satellite DNA of H has higher Tm and GC content than that of the PC and DC satellites. A 200 bp basic repeat unit from hypocotyl nuclear DNA has been cloned and characterised.